Project description:Diachasmimorpha longicaudata parasitoid wasps carry a symbiotic poxvirus, known as DlEPV, within the female wasp venom gland. We sequenced RNA from venom gland tissue to identify DlEPV orthologs for 3 conserved poxvirus core genes. The DlEPV ORFs identified from this transcriptome were used to design primers for downstream RT-qPCR analysis and RNAi knockdown experiments.
Project description:The purpose of this project was to identify venom proteins from the venom gland of Leptopilina heterotoma (strain Lh14), a parasitoid wasp species that infects fruit flies in the genus Drosophila.