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H3K9-butyryl (H3K9Bu) and H3K9-acetyi (H3K9ac) genome patterns before and after transverse aortic constriction in Balb/cJ and Balc/cByJ mice

ABSTRACT: H3K9-butyryl (H3K9Bu) and H3K9-acetyi (H3K9ac) genome patterns before and after transverse aortic constriction in Balb/cJ and Balc/cByJ mice

PROVIDER: PRJNA693852 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13499476.fastq.gz	Fastqsanger.gz
	SRR13499477.fastq.gz	Fastqsanger.gz
	SRR13499478.fastq.gz	Fastqsanger.gz
	SRR13499479.fastq.gz	Fastqsanger.gz
	SRR13499480.fastq.gz	Fastqsanger.gz

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H3K9-butyryl (H3K9Bu) and H3K9-acetyi (H3K9ac) genome patterns before and after transverse aortic constriction in Balb/cJ and Balc/cByJ mice

Project description:Balb/cJ mice on a low-fat diet show a decline in promoter H3K9-butyryl (H3K9Bu) in the heart, after subjecting them to transverse aortic constriction (TAC). In contrast, Balb/cByJ, which has a deletion in the ACADS gene, exhibit an increase in promoter H3K9Bu. Conversely, H3K9ac increases in the former and declines in the latter.

2021-05-11 | GSE165271 | GEO

The effect of transverse aortic constriction on gene expression in Balb/cJ versus Balb/cByJ(∆ACADS) mice hearts

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The effect of diet on H3K9-butyryl and H3K9-acetyl in the heart before and after transverse aortic constriction in C57Bl/6J mice

Project description:Mice (C57Bl/6J) that are fed increasing levels of dietary fat (0, 10, and 60 Kcal%) show a parallel increasing decline in global promoter-H3K9-butyryl (H3K9Bu), but none in promoter-H3K9-acetyl (H3K9ac), in the heart. Additionally, transverse aortic constriction (TAC) induces a further decline in H3K9Bu, compared to an increase in H3K9ac.

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Project description:We found BALB/cByJ mice showed enhanced sensitivity on the 53.5°C hot plate and mechanical stimulation in the von Frey test compared to BALB/cJ mice and replicated decreased gross brain weight in BALB/cByJ versus BALB/cJ. We then identified a quantitative trait locus (QTL) on chromosome 13 for hot plate sensitivity (LOD = 10.7; p < 0.001; peak = 56 Mb) and a QTL for brain weight on chromosome 5 (LOD = 8.7; p < 0.001). Expression QTL mapping of brain tissues identified H2afy (56.07 Mb) as the top transcript with the strongest association at the hot plate locus (FDR = 0.0002) and spliceome analysis identified differential exon usage within H2afy associated with the same locus. These data are spinal cord RNAseq to confirm differential expression of H2afy and other candidate genes.

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Single-Cell Genomics of Transverse Aortic Constriction in Mouse

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The effect of diet on H3K9-butyryl and H3K9-acetyl in the heart before and after transverse aortic constriction in C57Bl/6J mice

Project description:The effect of diet on H3K9-butyryl and H3K9-acetyl in the heart before and after transverse aortic constriction in C57Bl/6J mice

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Proteome wide screening for ubiquitination, ISGylation and NEDDylation sites in wildtype and ISG15 knockout mouse hearts after transverse aortic constriction

Project description:Hearts(left ventricles) were harvested from control wildtype and ISG15 knockout mice and wildtype and ISG15 knockout mice 4 weeks after pressure overload induced by transverse aortic constriction. Nano LC-MS/MS analysis was performedon left ventricle tissue following protein extraction, trypsin digestion, peptide desalting, anti K-e-GG enrichment.

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