Lens culinaris
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA694356 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Lentil transcriptional profiling in respoinse to Ascochyta infection
| PRJNA356810 | ENA
Project description:Transcriptional profiling of Ascochyta blight resistance in Lentil
| PRJNA106601 | ENA
Project description:Drought stress is one of the main environmental factors that affects growth and productivity of crop plants, including lentil. To gain insights into the genome-wide transcriptional regulation in lentil root and leaf under short- and long-term drought conditions, we performed RNA-seq on a drought-sensitive lentil cultivar (Lens culinaris Medik. cv. Sultan). After establishing drought conditions, lentil samples were subjected to de novo RNA-seq-based transcriptome analysis. The 207,076 gene transcripts were successfully constructed by de novo assembly from the sequences obtained from root, leaf, and stems. Differentially expressed gene (DEG) analysis on these transcripts indicated that period of drought stress had a greater impact on the transcriptional regulation in lentil root. The numbers of DEGs were 2915 under short-term drought stress while the numbers of DEGs were increased to 18,327 under long-term drought stress condition in the root. Further, Gene Ontology analysis revealed that the following biological processes were differentially regulated in response to long-term drought stress: protein phosphorylation, embryo development seed dormancy, DNA replication, and maintenance of root meristem identity. Additionally, DEGs, which play a role in circadian rhythm and photoreception, were downregulated suggesting that drought stress has a negative effect on the internal oscillators which may have detrimental consequences on plant growth and survival. Collectively, this study provides a detailed comparative transcriptome response of drought-sensitive lentil strain under short- and long-term drought conditions in root and leaf. Our finding suggests that not only the regulation of genes in leaves is important but also genes regulated in roots are important and need to be considered for improving drought tolerance in lentil.
2019-04-19 | GSE115199 | GEO
Project description:Lentils (Lens culinaris) are produced in diverse agroecological regions and are consumed as one of the most important food legumes at world-wide. Lentils possess a nutritional profile from a human health perspective that is not only nutrient-dense but also offers a better balance between protein and carbohydrates. However, lentil causes food allergy which has been a significant concern due to increased consumption in parts of the world. Len c3, a none-specific Lipid Transfer Protein (LTP), was identified as one of the allergens in lentil seeds. In this study, we firstly identified the Len c3 encoding gene LcLTP3b via peptide sequence blasting. We then focused on screening natural variations to search for lentil germplasms that harbors natural mutated allergen-encoding genes. A natural variation 11 was identified with mutations at LcLTP3b and low accumulation of vicilin through genomic-assisted approaches. Furthermore, we generated a pool of lentil germplasms with Len c3-free background through crossing the identified natural variation 11 with two lentil cultivars, CDC Redmoon and CDC Gold. These Len c3-free lentil germplasms can be used as a breeding resource targeting at reducing allergen risk in lentil consumption.
2024-02-29 | GSE255951 | GEO
Project description:Lens culinaris cultivar:Lentil Genome sequencing and assembly
| PRJNA916816 | ENA
Project description:A submergence tolerant indica rice cultivar FR13A, was also reported to withstand salt stress and proven in our experiments. The mechanism of tolerance is yet to be studied by forward genetics approach. However, it is known that salt stress tolerance is governed by several QTLs and not by a single gene. To understand the mechanism of such a complex mechanism of salt tolerance we selected, two indica rice genotypes namely, I) FR13A, a tolerant indica variety and ii) IR24, a susceptible genotype for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under constitutive and salt stress conditions at seedling stage. Keywords: Mechanism of salt tolerance
2007-12-21 | GSE6600 | GEO