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Identification of gene related to stem cells from human exfoliated deciduous teeth (SHED) into epithelial-like differentiation using co-culture in epithelial microenvironment

ABSTRACT: Identification of gene related to stem cells from human exfoliated deciduous teeth (SHED) into epithelial-like differentiation using co-culture in epithelial microenvironment

PROVIDER: PRJNA694633 | ENA |

REPOSITORIES: ENA

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Identification of gene related to stem cells from human exfoliated deciduous teeth (SHED) into epithelial-like differentiation using co-culture in epithelial microenvironment

Project description:We employed microarray profiling to determine the gene(s) that control human exfoliated deciduous teeth (SHED) differentiation into epithelial-like cells. We introduced epithelial microenvironment using keratinocyte serum free medium (KSFM) and co-culture with human epithelial keratinocyte (HEK001) to promote epithelial-like differentiation.

2024-02-01 | GSE165451 | GEO

Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

Project description:To explore the cellular response of stem cell derived from human exfoliated deciduous teeth (SHED) in response to inorganic pyrophosphate (PPi). SHED was extracted from human primary exfoliated teeth. The cellular responses including cell proliferation, apoptosis, migration, transcriptomic expression was investigated afterward PPi treatment at the distinct concentration. Additionally, Alizarin red, oil red O, and alcian blue staining were performed to evaluate the multipotency. Inorganic pyrophosphate (PPi) had no although effect on cell cycle, yet enhanced migratory cell, compared to control. Additionally, PPi abolished the gene expression of osteogenic genes and also calcium deposition. Interestingly, the inhibition of mineralization by PPi was not reversed though Pi3K and MAPK family, including ERK, P38, and JNK signalling pathway. On the contrary, PPi induced the ALPL and COL1A1 gene expression and reduced RANKL mRNA and protein in the condition medium, resulting in a decrease of osteoclast formation. The transcriptomic profiles illustrated PPi modulated interferon ⍺/ , TGF-β1, NOTCH signalling pathway, and metabolism of lipid. In summary, our study revealed that PPi enhanced migratory ability, however inhibited osteo/odontogenic, adipogenic and chondrogenic differentiation. This study implicated that PPi can modulate cellular responses of SHED.

2024-12-05 | GSE281644 | GEO

stem cells from human exfoliated deciduous teeth cultured with human platelet lysate

Project description:Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients, especially fetal bovine serum, will increase the risk of zoonotic disease transmission during the use of animal-derived products. Human-platelet-lysate (hPL) was used in human cell expansion with reliability in clinical applications. This study aimed to explore the feasibility and effect of HPL in the cultivation of SHED. We use proteomics to interpret the impact of HPL on the protein profile of SHED. Propagation, differentiation, qPCR and immunofluorescence were used to detect the effect of HPL on SHED.

2022-05-19 | PXD026687 | Pride

SHED: control vs Hepatocyte-like cells

Project description:Profiling of chromosomal gains and losses in a genomic complement of stem cells from human exfoliated deciduous teeth comparing control undifferentieated SHED with hepatocyte differentiated SHED.

2018-02-01 | GSE102974 | GEO

Transcriptome analysis of bFGF and WNT3A treated stem cells isolated from human exfoliated deciduous teeth

Project description:bFGF regulated stemness maintenance and cell differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). In addition, WNT3A is also shown to regulate osteogenic differentiation in mesenchymal stem cells. Here, transcriptome analysis of bFGF and WNT3A treated SHEDs was evaluated using a high throughput RNA sequencing technique. Results demonstrated that bFGF regulated genes related to cell cycle control and cell proliferation in SHEDs.

2020-07-07 | GSE131758 | GEO

Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

Project description:Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

| PRJNA1185148 | ENA

Single-cell transcriptome analysis of stem cells from human exfoliated deciduous teeth investigating functional heterogeneity in immunomodulation

Project description:Background: Mesenchymal stem cells (MSCs) have been widely used in the treatment of various inflammatory diseases. The inadequate understanding of MSCs and its heterogeneity can impact the immune environment maybe the cause of the good outcomes of MSCs-based therapy cannot be always achieved. Recently, stem cells from human exfoliated deciduous teeth (SHED) showed a great potential in inflammatory and autoimmune diseases due to its unique immunomodulation properties. However, the cellular heterogeneity and the corresponding immunomodulation functions of SHED remains unclear. Methods: In this study, we applied single-cell RNA sequencing (scRNA-seq) to analyze and performed bioinformatic analysis to clarify the variations immunomodulation functions of SHED subpopulations in different differentiation state. After the integration of public available databases, we analyzed the transcriptome and compared the difference of immunomodulation functions of MSCs from different tissues. Results: SHED in low differentiation state (S7) exhibited the powerful ability to recruit multiple types of immune cells, whereas SHED in relatively high differentiation state (S1) may hold strong ability to secret many factors with paracrine signaling capacity. Compared with human bone marrow derived mesenchymal stem cells (hBMSCs) or human umbilical cord-derived mesenchymal stem cells (hUCMSCs), SHED is stronger in immunomodulatory property, especially in recruitment of Th17, Th22 and Treg cells. Conclusion: SHED was a heterogeneous MSCs population with different differentiation states and immunomodulatory functions. SHED may have some advantages in treatment of inflammatory and autoimmune diseases. Our works provides some theoretical foundation for the SHED-based therapy in clinical applications.

2024-01-01 | GSE221216 | GEO

Effects of newly developed pulp-capping materials on early response of stem cells from exfoliated deciduous teeth: cell differentiation, migration, and proliferation based on next gene sequencing analysis

Project description:Aim : This study aimed to identify early effect of newly developed pulp capping materials on differentiation, migration, and proliferation of stem cells from exfoliated deciduous teeth (SHED) using next gen sequencing analysis. Methodology : SHED were cultured and exposed to MTA, Biodentine (BD), and Theracal (TC) for 24 and 72 h. WST assay was performed to evaluate cell viability. After total RNA was isolated, QuantSeq 3’ mRNA-Sequencing was performed to identify differential gene expression in MTA-, BD-, TC-exposed SHED. Bioinformatics analysis was also performed. Results : WST assay showed no significant difference in SHED viability among materials (p>0.05). In gene set enrichment analysis, hallmark gene set enrichment differed. Gene sets associated with epithelial mesenchymal transition and hedgehog signaling were enriched in all materials. In BD, gene sets with inflammatory response and Wnt/β-catenin signaling were enriched, whereas enrichment of gene sets with TNF-α signaling and apoptosis were identified in TC. Conclusions : Despite cell viability of SHED exhibited similar results among MTA, BD, and TC, patterns of gene networks differed, suggesting that diverse gene functional difference may be involved in these materials.

2020-04-17 | GSE139497 | GEO

Gene Profiles of Mesenchymal Stromal Cells from Different Tissue

Project description:Mesenchymal stromal stem cells from human bone marrow (BMSCs), adipose tissuse (ADSCs) were purchased from ATCC. Human exfoliated deciduous dental pulps were cultured in vitro and purified to get stem cells from human exfoliated deciduous teeth (SHED). Markers of MSCs were checked before scRNA seq.

2023-09-12 | GSE157863 | GEO

Comparative gene expression analysis of the human dental pulp in deciduous and permanent teeth

Project description:Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These differences are attributable to their genetic backgrounds. Therefore the purpose of this study is to compare the differences of dental pulp in deciduous and permanent teeth. Pulp samples were obtained from permanent premolars (n=6, aged 11-14 years) and deciduous teeth (n=6, aged 11-14 years). Comparative cDNA microarrary analysis revealed several differences in gene expression between the deciduous and permanent pulp tissues. Each GSM record represents a pulp sample pooled from two teeth samples.

2014-08-21 | E-GEOD-51449 | biostudies-arrayexpress

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