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High-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

ABSTRACT: high-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

PROVIDER: PRJNA694943 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13532207_1.fastq.gz	Fastqsanger.gz
	SRR13532207_2.fastq.gz	Fastqsanger.gz
	SRR13532208_1.fastq.gz	Fastqsanger.gz
	SRR13532208_2.fastq.gz	Fastqsanger.gz
	SRR13532209_1.fastq.gz	Fastqsanger.gz

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high-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

Project description:We report the differential expression of circRNAs between T-BEAS-2B cells (cadmium-transformed BEAS-2B cells) and C-BEAS-2B cells (passage-matched control BEAS-2B cells) by high-throughput sequencing. T-BEAS-2B cells are BEAS-2B cells transformed by cadmium at 2.0 μM for twenty weeks, and C-BEAS-2B cells are their passage-matched control. RNAs were sequenced on Illumina HiSeq Xten platform in triplicates, and expressions of circRNAs were calculated by TPM (transcripts per kilobase of exon model per million mapped reads). Clean data per sample exceeds 10 GB. We find 235 significantly up-regulated circRNAs and 271 significantly down-regulated circRNAs in T-BEAS-2B cells relative to C-BEAS-2B cells. Our work provides clues and evidence for exploring the mechanism of circRNAs in cadmium carcinogenesis.

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