Project description:High-Density Genetic Map Construction and QTLs Identification for Heat Stress Tolerance in Chickpea Using Next-Generation Double-Digest Restriction-Site-Associated DNA Sequencing (ddRADseq)
Project description:Bioinformatics approaches for viral metagenomics in plants using short RNAs : model case of study and application to a Cicer arietinum population
Project description:The total RNA were extracted from pooled tissues of leaves and flowers from several plants of chickpea (Cicer arietinum) using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Then small RNAs ranging in 18–30 nucleotides were size fractionated electrophoretically, isolated from the gel, ligated with the 5′ and 3′ RNA adapters. The ligated product was reverse transcribed and subsequently amplified using 10–12 PCR cycles. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to predict microRNAs and phased small interfering RNAs from chickpea.
Project description:Transcriptional profiling of chickpea genes differentially expressed in response to drought stress using high density oligonucleotide microarray