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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

ABSTRACT: An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

PROVIDER: PRJNA695075 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13537408.fastq.gz	Fastqsanger.gz
	SRR13537409.fastq.gz	Fastqsanger.gz
	SRR13537410.fastq.gz	Fastqsanger.gz
	SRR13537411.fastq.gz	Fastqsanger.gz
	SRR13537412.fastq.gz	Fastqsanger.gz

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

Project description:An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (BSA-seq dataset)

Project description:An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (BSA-seq dataset)

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation (smallRNA-seq dataset)

Project description:Foreign DNA is silenced in plants by small RNA-directed DNA methylation (RdDM). RNA Polymerase V (Pol V) is the key protein required to recruit DNA methyltransferases to a RdDM target region. However, the mechanism required for the initial recruitment of Pol V to a targeted locus remains a mystery. In this study, we used first generation (T1) transgene transformations to elucidate a novel mechanism by which Pol V is recruited to both new transgenic and endogenous loci. This study is heavily dependent on analyzing the DNA methylation pattern and small RNAs produced from specific loci. All small RNA sequences (sRNA-seq) and bisulfite converted amplicon sequencing data (BSAS) used in this study are submitted as part of this data series.

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An siRNA-guided AGO protein recruits Polymerase V to initiate RNA-directed DNA methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-02 | GSE165575 | GEO

Ago-APP: a GW peptide-based Argonaute affinity capture strategy

Project description:During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of all Ago protein complexes expressed in diverse species to identify associated proteins, small RNAs or target mRNAs. We refer to our method as Ago protein Affinity Purification by Peptides (Ago-APP). Comparison of small RNA lengths in total RNA and APP-enriched RNA samples

2015-09-08 | E-GEOD-70553 | biostudies-arrayexpress

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AGO pathway

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smallRNA profiling of THP1 cell line

Project description:Analysis of smallRNA in THP1 (human monocytic leukemia) cell line in order to correlate miRNA activity with target abundance. THP1 smallRNA profiles were generated in triplicates by deep-sequencing in Illumina HiSeq2000.

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