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Expression data from Fibroblast-like Synoviocytes (FLS) isolated from patients with Juvenile Idiopathic Arthritis (JIA) - compares untreated FLS to FLS exposed to conditioned media from Chondrocytes (Ch)

ABSTRACT: Expression data from Fibroblast-like Synoviocytes (FLS) isolated from patients with Juvenile Idiopathic Arthritis (JIA) - compares untreated FLS to FLS exposed to conditioned media from Chondrocytes (Ch)

PROVIDER: PRJNA695234 | ENA |

REPOSITORIES: ENA

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Expression data from Fibroblast-like Synoviocytes (FLS) isolated from patients with Juvenile Idiopathic Arthritis (JIA) - compares untreated FLS to FLS exposed to conditioned media from Chondrocytes (Ch)

Project description:This dataset was used to determine the influence of conditioned media from Ch and how that influence affects genome-wide gene transcription in fibroblast-like synoviocytes In this dataset, we include data from untreated fibroblast-like synoviocytes and FLS cultured in chondrocyte-conditioned media, both normal FLS (C ) and JIA FLS (J).

2021-05-19 | GSE165626 | GEO

Identification of genes regulating TRAIL-induced apoptosis in rheumatoid arthritis fibroblasts-like synoviocytes (RA FLS)

Project description:Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease involving primarily the synovial membranes and articular structures of multiple joints. A hallmark of RA is the pseudo-tumoral expansion of fibroblast-like synoviocytes (FLS), as these cells invade and finally destroy the joint structure. RA FLS have been therefore proposed as a therapeutic target. > TNF-related apoptosis-inducing ligand (TRAIL) has been described as a pro-apoptotic factor on malignant cells. The fact that fibroblasts-like-synoviocytes (FLS) in rheumatoid arthritis RA patients exhibit tumor like features led us to investigate the effect of TRAIL on ex-vivo RA FLS. We have previously described that TRAIL induces apoptosis only in a subset of RA FLS, but an induction of proliferation in the surviving cells. This observation corresponds to the pleiotropic effects of TRAIL observed on primary human tumor cells. We also observed that sensitivity to TRAIL-induced apoptosis varied in RA FLS from one patient to another, and was correlated with disease severity. We therefore screened for genes that were differentially expressed in RA FLS sensitive and resistant to TRAIL induced apoptosis in order to understand molecular factors making cells resistant or sensitive to TRAIL induced apoptosis.

2018-02-12 | GSE65908 | GEO

Single cell sequencing of fibroblast-like synoviocytes (FLS) isolated from hind paws of Col6a1-cre Bmal1-flox PER2::LUC mice

Project description:Fibroblast-like synoviocytes (FLS) were isolated from the hind paws of Col6a1-cre Bmal1-flox PER2::LUC mice for single cell sequencing to determine the effect of Bmal1 knockout on FLS cell function. FLS cells were isolated from joint digests by selection for Podoplanin expression, then fixed and barcoded for single cell transcriptomic analysis using a split-pool ligation-based transcriptomic sequencing (SPLiT-seq) approach.

2024-06-06 | E-MTAB-13320 | biostudies-arrayexpress

Transcriptional responses to TNF following HFl treatment of primary rheumatoid arthritis fibroblast like synoviocytes (RA-FLS)

Project description:We report the effects of Hfol on TNF induction of inflammatory genes in wild type cells versus cells depleted of GCN2 RNA-seq analysis of primary rheumatoid arthritis fibroblast like synoviocytes (RA-FLS) treated with TNFa in the presence or absence of Halofuginone (HF).

2020-03-22 | GSE145204 | GEO

RNA-seq of mouse fibroblast-like synoviocytes (FLS) isolated from mice with collagen-induced arthritis (CIA) and naive littermates

Project description:Fibroblast-like synoviocytes (FLS) were isolated from the inflamed joints of mice with collagen-induced arthritis (CIA) or the joints of naive littermates to characterise gene expression changes in response to chronic inflammation. Cells were isolated at two times of day (zeitgeber time, ZT4 and ZT16) to characterise diurnal variation in inflammatory symptoms and responses. FLS cells were isolated from joint digests based on Podoplanin expression prior to RNA extraction and library preparation for sequencing.

2024-06-06 | E-MTAB-13317 | biostudies-arrayexpress

Murine fibroblast-like synoviocytes: Control vs Ad-Epas1 infected

Project description:Transcriptional profiling of mouse fibroblast-like synoviocytes (FLS) comparing FLS infected with empty adenovirus and Epas1 adenovirus. RNA was extracted from each FLS. We used microarrays to determine the effect of Epas1 overexpression on FLS and identifying the noble regulatory molecules during rheumatoid arthritic pathogenesis

2015-10-02 | GSE73658 | GEO

Transcription profiling of normal and Mucopolysaccharidosis type VI rat fibroblast-like synoviocytes

Project description:Gene expression in 9 month-old Mucopolysaccharidosis type VI rat fibroblast-like synoviocytes (FLS) were compared to age-matched normal rat FLS

2007-10-24 | E-TABM-306 | biostudies-arrayexpress

Expression data from fibroblast-like synoviocytes (FLS) transfected with mimic-miR-23b or mimic-NC

Project description:We found microRNA miR-23b was down-regulated in local inflammatory tissues of autoimmune disease such as RA, SLE and related mouse models such as CIA, lpr, EAE. Re-expression of miR-23b significantly inhibits autoimmune pathogenesis of CIA, Lpr and EAE. To identify potential targets of miR-23b, we use microarray gene-expression analysis to identify transcripts which could be repressed by miR-23b. RA: rheumatoid arthritis, CIA: Collagen-induced arthritis, SLE: systemic lupus erythematosus, EAE: experimental autoimmune encephalomyelitis We tansfected fibroblast-like synoviocytes (FLS) with mimic-miR-23b or mimic-NC.Cells were collected and total RNA was extracted for the Affymetrix GeneChipÂ® Human Genome U133 Plus 2.0 Array

2012-12-19 | E-GEOD-37427 | biostudies-arrayexpress

Human fibroblast-like synoviocytes (FLSs) -derived secretome

Project description:Invasive pannus, mainly composed of fibroblast-like synoviocytes (FLSs), is a hallmark of rheumatoid arthritis (RA) pathology. Secreted proteins from RA-FLS play key roles in RA invasive pannus. However, RA-FLS-derived secretome associated with invasive pannus has not been systematically investigated. Here, we first identified 843 secreted proteins from RA-FLSs treated with TNFα and IL-1β using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Functional enrichment analysis revealed that 58.5% (493 proteins) of the secretome were associated with pannus-driven RA pathologies. Among them, parallel reaction monitoring (PRM) analysis then identified 16 secreted proteins that were increased in RA SFs (117 samples) than in OA SFs (45 samples). Of them, MYH9 further showed significant positive correlations with RA pathological parameters, such as synovial hyperplasia, increased articular vascularity, and inflammation severity. Molecular and cellular experiments confirmed that MYH9 was expressed in RA-FLSs and more highly under disease-aggravating conditions, and MYH9 depletion significantly defected migration and invasion of RA-FLS in RA pannus. Our study provides a comprehensive resource of RA-FLS-derived secretome, and our results suggest MYH9 that was increased in RA SF and strongly correlated with disease severity as a potential therapeutic target for invasive pannus.

2023-06-30 | PXD041077 | Pride

Regulation of type I interferon signature by VGLL3 in the fibroblast-like synoviocytes of rheumatoid arthritis patients via targeting the Hippo pathway

Project description:To address the regulation of interferon-stimulated genes expression by VGLL3 in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) patients, VGLL3 was overexpressed in RA-FLS via a lentiviral vector. After four days of transfection, VGLL3-overexpressed RA-FLS and vector-transfected RA-FLS were subjected to RNA sequencing.

2022-08-17 | GSE200030 | GEO

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