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Rationally designed, self-assembly, multifunctional hydrogel depot repairs severe spinal cord injury

ABSTRACT: Rationally designed, self-assembly, multifunctional hydrogel depot repairs severe spinal cord injury

PROVIDER: PRJNA695250 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13562711_1.fastq.gz	Fastqsanger.gz
	SRR13562711_2.fastq.gz	Fastqsanger.gz
	SRR13562712_1.fastq.gz	Fastqsanger.gz
	SRR13562712_2.fastq.gz	Fastqsanger.gz
	SRR13562713_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Rationally designed, self-assembly, multifunctional hydrogel depot repairs severe spinal cord injury

Project description:Following severe spinal cord injury (SCI), dysregulated neuroinflammation causes neuronal and glial apoptosis, resulting in scar and cystic cavity formation during wound healing process and ultimately atrophic nerve regrowth inhibitory microenvironment. Because of this complex and dynamic nature of SCI pathophysiology, a systemic solution for scar and cavity free wound healing while remodeling microenvironment for nerve regrowth has been rarely explored. To address this challenge, we provided a one-step solution through a self-assembly, multifunctional hydrogel depot punctually releasing the anti-inflammatory drug methylprednisolone sodium succinate (MPSS) and growth factors locally according to its pathophysiology to repair severe SCI. We found release of growth factors alone could only provide some tissues/axons protection, but failed to eliminate all cystic cavity formation. Sustained release of MPSS is sufficient to modulate dysregulated neuroinflammation and eliminate almost all cystic cavity but unable to alleviate impenetrable scar boundaries, which are largely responsive for nerve regrowth failure. Strikingly, synergically releasing anti-inflammatory drugs MPSS and growth factors in the hydrogel depot along SCI pathophysiology protected spared tissues/axons from secondary injury, promoted scar boundary and cavity free wound healing and made permissive bridges for remarkable axonal regrowth. Behavior and electrophysiology studies indicated that the remnant of spared axons but not regenerating axons mediated functional recovery, strongly suggesting that additional interventions are still required to make the rebuilt neuronal circuits perform functions. Taken together, these findings pave the way to develop a systemic solution to treat acute SCI.

2022-01-01 | GSE165635 | GEO

In vivo analysis of spinal cord astrocyte and non-astrocyte gene expression after traumatic spinal cord injury, with or without intraspinal treatment with hydrogel depot containing FGF+EGF

Project description:Comparison of genomic data from astrocytes and non-astrocyte cells from mice with or without FGF+EGF after SCI. We conducted genome-wide RNA sequencing of (i) immunoprecipitated astrocyte-specific ribosome-associated RNA (ramRNA) and (ii) the non-precipitated (flow-through) RNA deriving from non-astrocyte cells, from spinal cord tissue of mice recieving i) SCI alone, ii) SCI+hydrogel depot containing FGF+EGF, or iii) SCI+empty hydrogel depot.

2018-09-20 | GSE111529 | GEO

Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects

Project description:The native microenvironment following osteochondral defect is not permissive for its endogenous regeneration. A variety of hydrogels have been developed to regulate cell behaviors; however, there is still a lack of hydrogels with multifunctional properties that can provide a supportive microenvironment for endogenous regeneration of osteochondral defects. In this study, a multifunctional polyphenol-based silk hydrogel was developed and its effectiveness on in situ osteochondral regeneration was investigated. Silk fibroin (SF) was crosslinked by tannic acid (TA) to form a hydrogen-bonded supramolecular network, followed by enzymatic crosslinking using horseradish peroxidase (HRP)/H2O2 to form a stable SF-TA hydrogel. In vitro investigations showed that the proposed SF-TA hydrogel exhibited a multitude of biological effects including scavenging reactive oxygen species (ROS), maintaining cell viability, and promoting the proliferation of bone marrow mesenchymal stem cells (BMSCs), as a result of the incorporation of the bioactive TA molecule. Besides, TA had multiple phenolic hydroxyl groups that formed interactions with the therapeutic molecule E7 peptide which improved BMSCs migration when continuously released from the SF-TA hydrogel. In vivo, after implantation for 12-weeks into a rabbit osteochondral defect, the SF-TA hydrogels promoted enhanced regeneration of both cartilage and subchondral bone as compared to hydrogels without TA incorporation. These findings indicated that the multifunctional SF-TA hydrogel provided a microenvironment suitable for the endogenous regeneration of osteochondral defects, thus the SF-TA hydrogel exhibited promising properties for future clinical applications.

2021-11-28 | PXD030026 |

Exploiting Synergistic Effect of Controlled Co-Delivery Medicine for Soft Tissue Transplantation using Double System of Bio-Multifunctional Hydrogel

Project description:Autologous skin flap transplantation is a common method to repair complex soft tissue defects caused by cancer, trauma, and congenital malformations, among other conditions; however, limited blood supply range and post-transplantation ischemia-reperfusion injury (IRI) can cause distal necrosis of the flap and loss of long-term function, severely limiting the indications for this type of surgery. Here, development of a bio-multifunctional hydrogel for programmatic co-delivery medicine, DCDM, that meets the blood supply requirements of skin flap transplantation and can deliver two types of gas molecules, CO and NO, in a timely manner and with a concentration gradient during surgery, is described. DCDM maintains the immediate opening of blood flow channels in transplanted tissue and effective blood perfusion throughout the perioperative period, activating perfusion of the hemodynamic donor site. These results demonstrate that DCDM promotes distal vascularization and long-term functional reconstruction of transplanted tissues by inhibiting inflammatory damage and accelerating blood vessel formation. The findings presented here are applicable to the extended fields of large soft tissue defect repair and transplant of various organs.

2022-06-09 | GSE205580 | GEO

A rationally designed JAZ subtype-selective agonist of jasmonate perception

Project description:A rationally designed JAZ subtype-selective agonist of jasmonate perception

| PRJNA434702 | ENA

Exploiting Synergistic Effect of Controlled Co-Delivery Medicine for Soft Tissue Transplantation using Double System of Bio-Multifunctional Hydrogel

Project description:Exploiting Synergistic Effect of Controlled Co-Delivery Medicine for Soft Tissue Transplantation using Double System of Bio-Multifunctional Hydrogel

| PRJNA846493 | ENA

Self-assembly of nanomicelles with rationally designed multifunctional building blocks for synergistic chemo-photodynamic therapy.

Project description:Rationale: The combination of photosensitizers, oxygen supply agents, and adjuvant therapy drugs in a single nano-drug delivery system for photodynamic therapy (PDT) has been showing great promises to overcome the inherent challenges of PDT for tumor treatment. However, the complicated preparation of integrating multiple components hampers their further developments. Here, we describe a self-assembly nanomicelle with rationally designed building blocks, which shows a high efficiency of synergistic chemo-photodynamic therapy in the animal modal. Methods: The nanomicelle was prepared by a coordination-driven self-assembly based on a rationally designed ferrocene cyclopalladated compound coupled with photosensitizers and hyaluronic acid (referred to as FCP-Tph/HA). The morphology, targeting drug delivery, pharmacokinetics, hemolysis, and multimodal synergistic therapy of FCP-Tph/HA were investigated. Results: The formation of nanomicelles presents a low hemolysis rate and a prolonged blood circulation time. FCP-Tph/HA possesses an enhanced antitumor effect in vitro through the specific binding of HA to CD44 and combining chemotherapy with oxygen self-supplying PDT. Simultaneously, the nanomicelle facilitates a significantly improved antitumor efficacy (>90% tumor regression) on a breast cancer model in vivo. Conclusion: Our results present a modular self-assembled nanomicellar platform with synergistic chemo-photodynamic therapy for challenging PDT-based tumor treatment.

| S-EPMC8899564 | biostudies-literature

Skin proteomics reveals the potential mechanism of AgNCs-hydrogel in promoting the regeneration of diabetic infectious wounds

Project description:Diabetic infectious wounds treatment is challenging due to insistent wound infections. Treating such complicated pathological diabetic infectious wounds needs to develop multifunctional materials and understand their mechanism. Here, we developed a novel material termed AgNCs-hydrogel, which is a multifunctional DNA hydrogel which dressing by integrating antibacterial silver nanoclusters. AgNCs-hydrogel was used for promoting the regeneration of diabetic infectious wounds in mouses because that it exhibited superior antibacterial activity, satisfactory biocompatibility, and effective ROS-scavenging property. Based on the skin proteomics, we explored the potential mechanism of AgNCs-hydrogel in treating mouse skin wounds. We found that AgNCs-hydrogel can accelerate proliferation and extracellular matrix (ECM) formation. In proteomic level, AgNCs-hydrogel can regulate some key proteins which mainly located in the extracellular exosome, involved in negative regulation of apoptotic process, performed ATP binding for accelerating diabetic infected wound closure. Therefore, this study provided a multifunctional material AgNCs-hydrogel and revealed its potential mechanisms in promoting the regeneration of diabetic infectious wounds.

2023-08-07 | PXD044438 |

Sequencing data for single cells and DNA-functionalized microbeads encapsulated in hydrogel droplets.

Project description:DNA barcodes can be used to identify single cells in a sequencing data space while optical codes can be used to track single live cells in an image data space. We have developed dual image and DNA (ID)-coding, which identifies individual single cells in both live image and sequencing data spaces. Samples provided here are relevant to proof-of-concept studies of ID-coding presented in the associated publication. DNA barcoded micro-particles were encapsulated in hydrogel droplets with or without single cells. The hydrogel droplets were then subjected to “single-droplet sequencing” where whole polyA-bearing nucleic acid components within a hydrogel droplet (i.e. mRNA from cells and synthetic DNA on beads) were concatenated by the same cell barcodes.

2023-12-11 | E-MTAB-12988 | biostudies-arrayexpress

Brown Adipose Tissue (BAT) in Visceral Fat Depot

Project description:Case story. A patient with massive infiltration of the visceral adipose tissue depot by BAT in a patient with a catecholamine secreting paraganglioma. BAT tissue was identified by protein expression of UCP1 (western blotting and immunostaining) The goal of the study is to identify patterns of gene expression in BAT containing visceral fat compared to the patient's own subcutanous fat which did not express BAT. For comparison a pool of mRNA isolated from visceral fat from obese subjects was used. Patient Case, Gene expression array from a biopsy from the patient's visceral fat and a biopsy from the subcutaneous fat compared to one array of mRNA from the visceral depot pooled from a group of obese subjects

2014-08-12 | E-GEOD-49795 | biostudies-arrayexpress

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