Ontology highlight
ABSTRACT:
PROVIDER: PRJNA695499 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13613000_1.fastq.gz | Fastqsanger.gz | |||
| SRR13613000_2.fastq.gz | Fastqsanger.gz | |||
| SRR13613001_1.fastq.gz | Fastqsanger.gz | |||
| SRR13613001_2.fastq.gz | Fastqsanger.gz | |||
| SRR13613002_1.fastq.gz | Fastqsanger.gz |
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Project description:This study aims to elucidate the impact of gut microbiota alterations on tumorigenesis and immune response in lung adenocarcinoma (LUAD). Using Gprc5a-/- mice as a model, we performed fecal microbiota transfer (FMT) from Gprc5a-/- and Gprc5a-/-; Lcn2-/- donors to investigate the role of gut microbiome changes in modulating tumor growth and the immune microenvironment. Single-cell RNA sequencing (scRNA-seq) was conducted on colonic lamina propria and subcutaneous tumor tissues. Our findings demonstrate that gut microbiota from Lcn2-deficient mice promotes systemic inflammation and immunosuppression, enhancing tumor progression. This study provides insights into the microbiome's influence on LUAD and potential therapeutic strategies targeting microbiome-related pathways.
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