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RNA Sequencing Analysis of MC38 tumors with Glutamate decarboxylase 1 Knockdown

ABSTRACT: RNA Sequencing Analysis of MC38 tumors with Glutamate decarboxylase 1 Knockdown

PROVIDER: PRJNA698537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13594944_1.fastq.gz	Fastqsanger.gz
	SRR13594944_2.fastq.gz	Fastqsanger.gz
	SRR13594945_1.fastq.gz	Fastqsanger.gz
	SRR13594945_2.fastq.gz	Fastqsanger.gz
	SRR13594946_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing Analysis of MC38 tumors with Glutamate decarboxylase 1 Knockdown

Project description:Various types of cancers, including colorectal cancer, have shown an unusual dependence on glutamine. Glutamine can undergo an alternate fate in neurons: conversion into a non-proteinogenic amino acid gamma-aminobutyric acid (GABA) by the glutamic acid decarboxylase (GAD), including two family members GAD1 and GAD2. Some studies have shown that GAD1 expression is dysregulated in certain cancer types, but the effect of GAD1 on the tumorigenic process remain largely unknown. Thus, we use mouse colon adenocarcinoma MC38 tumor samples to perform RNA-sequencing (RNA-seq) assay. The goal of this study is to investigate GAD1-mediated transcriptome changes within tumors.

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