Project description:The total RNA were extracted from tissues of roots from several plants of Panax notoginseng under CK and Cd stress treatment by using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to study of Panax notoginseng transcriptome under CK and Cd stress treatment.
Project description:Cupriavidus metallidurans CH34 is a metal resistant beta-proteobacterium. The genome of this bacterium contain many genes involved in heavy metal resistance. Gene expression of C. metallidurans was studied after the addition of of Zn(II), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II) or Co(II). Keywords: Heavy metal stress response Cultures of C. metallidurans CH34 were grown at 30°C until OD reached 0.6 (mid- exponential phase cultures). Heavy metals (0.8 mM of Zn(II), 0.5 mM of Cd(II), 0.1 mM of Cu(II), 0.6 mM of Ni(II), 0.4 mM of Pb(II), 5 uM of Hg(II) and 0.5 mM of Co(II)) were added to the culture for 30 minutes induction time. Total RNA was extracted, reverse-transcribed and labeled with Cy3-dCTP for the control (without metal) and with Cy5-dCTP for each conditions (challenged with one metal). Labeled cDNA were (control and one condition) added to a spotted slide for overnight hybridization at 42°C. Slides were scanned with a laser at 532 and 635 nm.
Project description:Cupriavidus metallidurans CH34 is a metal resistant beta-proteobacterium. The genome of this bacterium contain many genes involved in heavy metal resistance. Gene expression of C. metallidurans was studied after the addition of of Zn(II), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II) or Co(II). Keywords: Heavy metal stress response
Project description:Comparative RNA-Seq profiling of Sideroxydans sp. CL21, a microaerophilic, Fe(II)-oxidizer, and the facultative anaerobe Shewanella oneidensis, an Fe(III) reducer. The microorganisms were grown in co-culture and monoculture batch incubations under microaerobic growth conditions. RNA-Seq profiling was used to compare the transcriptomes of both Sideroxydans sp. CL21 and S. oneidensis when grown in co-culture compared to growth in monoculture
Project description:Purpose: In order to explore the detoxification mechanism of nitrogen against cadmium stress in plants Methods:Cultivation and treatment for four months of P.yunnanensis seedlings by soil culture, in triplicate, qRT–PCR validation was performed using TaqMan and SYBR Green assays Results:Exogenous nitrogen induced the expression of resistance genes Conclusions: Our study represents that exogenous nitrogen addition under Cd stress induced more differential expressed TFs.