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ABSTRACT: Phaeodactylum tricornutum sequencing

PROVIDER: PRJNA699705 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR13660264_1.fastq.gz	Fastqsanger.gz
	SRR13660264_2.fastq.gz	Fastqsanger.gz
	SRR13660265_1.fastq.gz	Fastqsanger.gz
	SRR13660265_2.fastq.gz	Fastqsanger.gz
	SRR13660266_1.fastq.gz	Fastqsanger.gz

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Project description:Loss of DNA methylation is traditionally associated with transcriptional up-regulation of transposable elements. Here we describe for the first time expression changes upon loss of DNA methylation in diatoms using Phaeodactylum tricornutum as a model species. The loss of DNA methylation was obtained by generation of DNMT5 KOs, a divergent DNMT with SNF-like domains. Expression analysis confirm the loss of DNA methylation is associated with de-repression of transposable elements in Phaeodactylum tricornutum with indirect effects on protein coding genes.

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Next generation sequencing for understading the role of nitrate reductase in remodeling intermediate metabolism in Phaeodactylum tricornutum

Project description:We have done next generation sequencing of optically thin, exponentialy growing WT Phaeodactylum tricornutum and our ~50% nitrate reductase knock-down cultures. Culture were grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation).

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Bioinspired cell silicification of the model diatom Phaeodactylum tricornutum and its effects on cell metabolism

Project description:Biosilicification enhances the mechanical strength and chemical stability of organisms. Diatoms are the natural model for studying cell silicification, with the model diatom Phaeodactylum tricornutum being known as the only species that could transition from non-silicified cells to silicified cells under environmental stress. In this study, single-cell sequencing was employed to investigate the wild-type P. tricornutum strain (WT-Pt) without cell silicification and the engineered strain (SG-Pt) with silicified cells. Our results indicate that SG-Pt exhibits clearly cellular clustering and enhanced iron metabolic function compared to WT-Pt.

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