Project description:To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing.
2025-05-27 | GSE262357 | GEO
Project description:pUC19 plasmid
| PRJNA596757 | ENA
Project description:Custom-primed rolling circle amplicons for highly accurate nanopore
| PRJNA1157368 | ENA
Project description:Rolling Circle Reverse Transcription Enables High Fidelity Nanopore Sequencing of Small RNA
Project description:We report isoCirc, a long-read sequencing strategy coupled with an integrated computational pipeline to characterize full-length circular RNA (circRNA isoforms) using rolling circle amplification (RCA) followed by long-read sequencing. Applying isoCirc to 12 human tissues, we determined full-length structures and examined tissue specificities of circRNA isoforms in human transcriptomes.
Project description:Circular RNAs (circRNAs) exhibit extended stability and enhanced,sustained protein expression. Nucleoside modification and rolling circle translation are supposed to reduce the immunogenicity and enhance the translational efficiency of circRNAs. However, it remains challenging to produce encodable circRNAs with modified nucleosides rather than the original nucleosides. Here, we identified a 39-nucleotide Cap-independent Translation Enhancer (CITE) element, termed BBV, which effectively drove the translation of nucleoside-modified RNAs with minimal immunogenicity. Subsequently, we demonstrated that the in vivo-produced circRNA harboring BBV could achieve efficient and sustained rolling circle translation lasting two weeks. Furthermore, we developed an extracellular vesicle-based delivery platform, termed EPM-EABR ASsisted Vesicle Yield (EASY), to deliver circRNAs without introducing viral Gag-Pol proteins. Additionally, we developed a Permuted Intron for Clean CircRNAs (PICC) system to produce scarless circRNAs via in vitro transcription and achieved efficient rolling circle translation driven by BBV. In a murine model, we demonstrated that the PICC-produced circRNA vaccine outperformed the N1mψ-mRNA vaccine in inducing anti-tumor immunity. Importantly, we succeeded to achieve complete substitution with modified nucleosides in circRNAs via Twister ribozyme self-cleaving, dramatically reducing the immunogenicity of circRNAs, but still retaining the ability of rolling circle translation. Collectively, we established a nucleoside-modified circRNA platform that facilitates efficient translation with minimal immunogenicity, offering a safer and more effective platform for applications in vaccine development and disease treatment.
2026-02-28 | GSE303451 | GEO
Project description:Rolling circle of Yeast RNAP mutants
| PRJNA923308 | ENA
Project description:Paramecium tetraurelia rolling-circle at different temperatures
| PRJNA839092 | ENA
Project description:Rolling-circle of C. elegans RNAPII mutants