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Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle

ABSTRACT: Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle

PROVIDER: PRJNA700585 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13651461_1.fastq.gz	Fastqsanger.gz
	SRR13651461_2.fastq.gz	Fastqsanger.gz
	SRR13651462_1.fastq.gz	Fastqsanger.gz
	SRR13651462_2.fastq.gz	Fastqsanger.gz
	SRR13651463_1.fastq.gz	Fastqsanger.gz

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Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle

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Project description:Myofibres (primary and secondary myofibre) are the basic structure of muscle and the determinant of muscle mass. To explore the skeletal muscle developmental processes from primary myofibres to secondary myofibres in pigs, we conducted an integrative three-dimensional structure of genome and transcriptomic characterization of longissimus dorsi muscle of pig from primary myofibre formation stage [embryonic Day 35 (E35)] to secondary myofibre formation stage (E80). In the hierarchical genomic structure, we found that 11.43% of genome switched compartment A/B status, 14.53% of topologically associating domains are changed intradomain interactions (D-scores) and 2,730 genes with differential promoter-enhancer interactions and (or) enhancer activity from E35 to E80. The alterations of genome architecture were found to correlate with expression of genes that play significant roles in neuromuscular junction, embryonic morphogenesis, skeletal muscle development or metabolism, typically, NEFL, MuSK, SLN, Mef2D and GCK. Significantly, Sox6 and MATN2 play important roles in the process of primary to secondary myofibres formation and increase the regulatory potential score and genes expression in it. In brief, we reveal the genomic reorganization from E35 to E80 and construct genome-wide high-resolution interaction maps that provide a resource for studying long-range control of gene expression from E35 to E80.

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