Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b at E9.0 days of development, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.
Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b at E9.0 days of development, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Four wild-type and four mutant samples, consisting of 3-4 embryos each, were used.
Project description:Neural crest cells are migratory progenitor cells that contribute to nearly all tissues and organs throughout the body. Their formation, migration and differentiation are regulated by a multitude of signaling pathways, that when disrupted can lead to disorders termed neurocristopathies. While work in avian and amphibian species has revealed essential factors governing the specification and induction of neural crest cells during gastrulation and neurulation in non-mammalian species, their functions do not appear to be conserved in mice, leaving major gaps in our understanding of neural crest cell formation in mammals. Here we describe Germ Cell Nuclear Factor (GCNF/Nr6a1), an orphan nuclear receptor, as a critical regulator of neural crest cell formation in mice. Gcnf null mutant mice, exhibit a major disruption of neural crest cell formation. The purpose of this experiment is to examine gene expression changes in response to Gcnf mutation in E9.0 mouse embryos.
Project description:We compared gene expression differences in the polytypic species complex Mus musculus (Mus musculus musculus, Mus musculus domesticus, Mus musculus castaneus and Mus musculus ssp) with that of Mus spretus via oligonucleotide microarrays representing more than 20,000 genes. Analysis of the results by two way ANOVA statistics suggests that the most genes with significant differences in expression levels among the subspecies are found in liver and kidney and the least in testis. This picture is different when one compares with Mus spretus, where the largest number of differences is found in testis. Keywords: multi-species comparison
Project description:Quantitative label free proteomics on an n=1 experiment of isolated tritosomes (lysosomes) from mus musculus, WT and a knock out of the LIMP2 protein.
Project description:Terminal differentiation of epidermal cells in Drosophila embryos requires the activity of a transcription factor. Svb is necessary and sufficient to induce this process. pri is a regulator of Svb activity, converting it from a repressor into an activator. To characterize the downstream Svb and pri effectors in cell morphogenesis, we performed microarrays in wt, svb -/- (no gene) and pri -/- (svb repressor) mutant conditions.
Project description:Transcriptome analysis of testicular cells in VRK1+/+ and VRK1-/- Mus musculus Gene expression in whole testicular cells from wild type (VRK1+/+) and VRK1-/- mutant Mus musculus, respectively, was measured. Four independent experiment for wild type and mutant, respectively, were performed.