Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Isolating and Cryo-Preserving Pig Skin Cells for Single Cell RNA Sequencing Study

ABSTRACT: Isolating and Cryo-Preserving Pig Skin Cells for Single Cell RNA Sequencing Study

PROVIDER: PRJNA701270 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13679870.fastq.gz	Fastqsanger.gz
	SRR13679871.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Isolating and Cryo-Preserving Pig Skin Cells for Single Cell RNA Sequencing Study

Project description:The pig skin architecture and physiology are similar to these of humans. Thus, the pig model is valuable for studying skin biology and testing therapeutics for skin diseases. The single-cell RNA sequencing technology allows quantitatively analyzing cell types, cell states, signaling, and receptor-ligand interactome at single-cell resolution and at high throughput. scRNA-Seq has been used to study mouse and human skins. To maximize the use of pig skin as a model system to study skin biology, we described a robust method for isolating and cryo-preserving pig single cells for scRNA-Seq. We showed that pig skin could be efficiently dissociated into single cells with high cell viability using the Miltenyi Human Whole Skin Dissociation kit and the Miltenyi gentleMACS Dissociator. In addition, we showed that the subsequent single cells could be cryopreserved using DMSO without causing additional cell death, cell aggregation, or changes in gene expression profiles. Using the developed protocol, we were able to identify all the major skin cell types. The protocol and results from this study will be very valuable for the skin research scientific community.

2021-02-11 | GSE166561 | GEO

Comprehensive transcriptional analysis of pig facial skin development

Project description:To study the development of pig facial skin after birth, we use the facial skin tissues of healthy Chenghua sows as experimental materials. we then performed gene expression profiling analysis using data obtained from RNA-seq of pig facial skin tissues at four time points.

2023-05-10 | GSE231573 | GEO

Pig Skin & Wound Microbiome

Project description:Pig Skin & Wound Microbiome

| PRJNA1055114 | ENA

Isolating and cryopreserving pig skin cells for single-cell RNA sequencing study.

Project description:The pig skin architecture and physiology are similar to those of humans. Thus, the pig model is very valuable for studying skin biology and testing therapeutics. The single-cell RNA sequencing (scRNA-seq) technology allows quantitatively analyzing cell types, compositions, states, signaling, and receptor-ligand interactome at single-cell resolution and at high throughput. scRNA-seq has been used to study mouse and human skins. However, studying pig skin with scRNA-seq is still rare. A critical step for successful scRNA-seq is to obtain high-quality single cells from the pig skin tissue. Here we report a robust method for isolating and cryopreserving pig skin single cells for scRNA-seq. We showed that pig skin could be efficiently dissociated into single cells with high cell viability using the Miltenyi Human Whole Skin Dissociation kit and the Miltenyi gentleMACS Dissociator. Furthermore, the obtained single cells could be cryopreserved using 90% FBS + 10% DMSO without causing additional cell death, cell aggregation, or changes in gene expression profiles. Using the developed protocol, we were able to identify all the major skin cell types. The protocol and results from this study are valuable for the skin research scientific community.

| S-EPMC8853494 | biostudies-literature

skin metagenome

Project description:Pig skin microbiota Metagenome

| PRJNA231552 | ENA

Comprehensive transcriptional analysis of pig facial skin development

Project description:Comprehensive transcriptional analysis of pig facial skin development

| PRJNA966966 | ENA

Isolating metastatic cell populations from zebrafish

Project description:Isolating metastatic cell populations from zebrafish

| PRJNA641651 | ENA

Salmonella pig phage study

Project description:Salmonella pig phage study

| PRJEB50190 | ENA

Repeat tick exposure elicits distinct immune responses in guinea pigs and mice [guinea pig]

Project description:RNA-seq gene expression analysis of guinea pig and mouse skin after tick bites

2023-04-20 | GSE148967 | GEO

RNA isolation from mouse skin biopies

Project description:Study to optimize our protocol for isolating RNA from skin biopsies from (hairless) SKH mice using different-diameter biopsy punches. Some mice were also treated with UVB radiation to check its effect on RNA yield. 4 mice total: 2 were irradiated with 300J/m2 UVB and 2 were non-irradiated. Post-mortem skin biopsies with 1.5mm, 2.0mm, and 2.5mm diameter punches were taken from the dorsal region.

2011-10-04 | E-GEOD-28463 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data