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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk [scRNA-seq]

ABSTRACT: Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk [scRNA-seq]

PROVIDER: PRJNA701277 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13680944.fastq.gz	Fastqsanger.gz

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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk.

Project description:Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk.

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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk

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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk

Project description:We report bulk RNA-sequencing for synchronized adult Sertoli cells in germ cell-sufficient (RFPxAMH-Cre) and germ cell-deficient (NANOS2 KO) mice. We also have single-cell RNA-sequencing data for RFPxAMH-Cre sorted Sertoli cells from unsynchronized adult testes (not included in this series).

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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk [scRNA-seq]

Project description:We report single-cell RNA-sequencing data for RFPxAMH-Cre sorted Sertoli cells from unsynchronized adult testes.

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Two distinct Sertoli cell states are regulated via germ cell crosstalk†.

Project description:Sertoli cells are a critical component of the testis environment for their role in maintaining seminiferous tubule structure, establishing the blood-testis barrier, and nourishing maturing germ cells in a specialized niche. This study sought to uncover how Sertoli cells are regulated in the testis environment via germ cell crosstalk in the mouse. We found two major clusters of Sertoli cells as defined by their transcriptomes in Stages VII-VIII of the seminiferous epithelium and a cluster for all other stages. Additionally, we examined transcriptomes of germ cell-deficient testes and found that these existed in a state independent of either of the germ cell-sufficient clusters. Altogether, we highlight two main transcriptional states of Sertoli cells in an unperturbed testis environment, and a germ cell-deficient environment does not allow normal Sertoli cell transcriptome cycling and results in a state unique from either of those seen in Sertoli cells from a germ cell-sufficient environment.

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Two Distinct Sertoli Cell States are Regulated via Germ Cell Crosstalk.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-31 | GSE166594 | GEO

Sertoli cell specific testicular gene expression of Connexin 43 knockout mice

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Sertoli cell specific testicular gene expression of Connexin 43 knockout mice

Project description:Infertility observed in adult Sertoli cell (SC)-specific Connexin 43 Knockout-mice rather seems to be an effect of the disturbed SC-Germ cell (GC) crosstalk than a direct consequence of the loss of Cx43 protein in SC with important GC specific genes being mostly affected by this deletion. Identification of differentially expressed genes in testis of cx43 KO-mice at developmental stage 8 days post partum when compared with testis of WT-mice

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Riding the spermatogenic wave: Profiling gene expression within neonatal germ and Sertoli cells during a synchronized initial wave of spermatogenesis

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Project description:Sertoli cells are highly polarized testicular cells that provide a nurturing environment for germ cell development and maturation during spermatogenesis. The Class III PI3K, PIK3C3/VPS34 plays key roles in endosomal membrane traffic and macroautophagy in various cell types; however, its role in Sertoli cells remains unclear. Here, we generated a mouse line in which Pik3c3 was specifically deleted in Sertoli cells (cKO) and found that after one round of normal spermatogenesis, the cKO mice quickly became infertile and showed disruption of Sertoli cell polarity, loss of germ cells and impaired spermiogenesis. Subsequent proteomics and phosphoproteomics analyses enriched the F-actin cytoskeleton network involved in the disorganized Sertoli-cell structure in cKO testis, and showed significantly increased expression of the F-actin negative regulator SCIN and reduced phosphorylation of the α-tubulin deacetylase HDAC6. Our results further demonstrated that the accumulation of SCIN in cKO Sertoli cells caused the disassembly of the F-actin cytoskeleton, which was related to the failure of SCIN degradation through the autophagy-lysosome pathway. Additionally, we found that the phosphorylation of HDAC6 at site S59 by PIK3C3 was essential for its degradation through the ubiquitin-proteasome pathway. As a result, the HDAC6 that accumulated in cKO Sertoli cells deacetylated SCIN at site K189 and led to a disorganized F-actin cytoskeleton. Taken together, our findings elucidate the indispensable role of PIK3C3 in maintaining Sertoli cell polarity and reveal a new mechanism in which both its protein kinase activity and its regulation of autophagy are required for the stabilization of the actin cytoskeleton.

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