Project description:To examine the effects of Tet3-inactivation on genome-wide transcriptional initiation, we determined the binding profiles of Pol II pSer5, a specific form of Pol II that marks transcriptional initiation sites, by ChIP-seq after DRB-induced block of transcriptional elongation.
Project description:We generated genome-wide chromatin-state maps of human airway smooth muscle cells. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, Lysine 4 monomethylation was used to highlight the location of potential regulatory domains in these cells.
Project description:We generated genome-wide maps of Pol II and Flag-LARP7 in human HeLa cells. We find that Flag-LARP7 and Pol II co-locolize on Pol II-specific sn/snoRNA genes.
Project description:We compared BRG1 binding and histone modification states between siControl and siBAF60a transfected human aortic smooth muscle cells (HASMCs) through ChIP sequencing.
Project description:We compared BRG1 binding and 4 histone modification states between siControl and siBAF60c transfected human aortic smooth muscle cells (HASMCs) through ChIP sequencing (ChIP-seq).