Project description:We report an small RNA sequencing (sRNA-seq) approach to identify host sRNAs involved in the nitrogen fixing symbiosis between Mesoamerican Phaseolus vulgaris and Rhizobium etli strains with different degrees in nodulation efficiency. This approach identified conserved and known microRNAs (miRNAs) differentially accumulated in Mesoamerican P. vulgaris roots in response to a highly efficient strain, to a less efficient one or to both strains.
Project description:The GOGAT (gltB) supressor strain RU2386 was compared to the wildtpe Rhizobium leguminosarum biovar viciae strain Rlv3841 after growth overnight on glucose aspartate.
Project description:The wiltype strain Rhizobium leguminosarum Rlv3841 was compared to the gltB mutant RU2307. Both strains were grown overnight on glucose (10 mM) aspartate (10 mM) AMS.