Project description:Mouse serum exosomes were harvested from OCExoInhib-treated mice or vehicle-treated mice at 2w after anterior cruciate ligament transection (ACLT). The exosomes were lysated and processed by Exosome RNA Column Purification Kit (System Biosciences). The miRNA were profiled using the Exosomal microRNA Amplification and Profiling Kit (System Biosciences)
Project description:Mouse serum exosomes were harvested from osteoclast-exosome blockage (OCExo-BLK) mice or osteoclast-exosome intact (OCExo-Intact) mice at 2w after anterior cruciate ligament transection (ACLT). The exosomes were lysated and processed by Exosome RNA Column Purification Kit (System Biosciences). The miRNA were profiled using the Exosomal microRNA Amplification and Profiling Kit (System Biosciences)
Project description:Human serum samples were collected from patients with history of three or more recurrent spontaneous abortion and non- pregnant women with two or more successful deliveries. Chlamydia trachomatis infection was detected in the samples collected from the patients and real time PCR was perform to study the expression of microRNA genes in these patients.
Project description:Human EMT PCR array Real-time quantitative PCR analysis of human EMT genes in PCR array data for expression of EMT genes in MCF-7 cells, upon treatment with exosomes released from TSP5-overexpressing human primary adipocytes (ND; ABM-007), compared to exosomes released from matched control adipocytes transduced with control lentiviral vector [Data S5] PCR array data for expression of EMT genes in MCF-7 cells after treating with TSP5 overexpressing cells' exosomes