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ATAC-seq for E14 cells knockdown with siRNA or shRNA

ABSTRACT: ATAC-seq for E14 cells knockdown with siRNA or shRNA

PROVIDER: PRJNA703640 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21824571_1.fastq.gz	Fastqsanger.gz
	SRR21824571_2.fastq.gz	Fastqsanger.gz
	SRR21824572_1.fastq.gz	Fastqsanger.gz
	SRR21824572_2.fastq.gz	Fastqsanger.gz
	SRR21824573_1.fastq.gz	Fastqsanger.gz

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ATAC-seq for E14 cells knockdown with siRNA or shRNA

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E14 cells knockdown with siRNA or shRNA

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E14 cells knockdown with siRNA or shRNA

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Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophages Transcriptomes for Acly-E14 siRNA effects

Project description:Purpose: The purpose of this study is to detect activated or silenced genes during bone marrow derived macrophages (BMDMs) transfected with control siRNA or Acly-E14 siRNA. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. BMDMs were transfected with control siRNA or Acly-E14 siRNA. 48 hour later, they were stimulated with LPS (100ng/ml) for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between BMDMs transfected with the indicated siRNAs.

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ATAC-seq and H4K16ac ChIP-seq profiles of E14 ES cells treated with cycloheximide

Project description:ATAC-seq and H4K16ac ChIP-seq profiles of E14 ES cells before and after treatment with cycloheximide (CHX, 1 ug/ml) for 3 hours.

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Effects of knockdown of ERK5 using PROTACs and siRNA on HCT116 cells

Project description:Changes in the cellular transcriptome, proteome and phosphorylated proteome after knockdown of ERK5 protein in HCT116 cells using PROTACs and siRNA, respectively.

2022-08-23 | PXD042635 |

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