Project description:We performed ATAC-sequencing in LSK cells (Lin(neg)/c-Kit(+)/Sca-1(+)) from shRNA mice carrying an shRNA for either Renilla or Stag2. ATAC-sequencing control (Renilla) and Stag2 knockdown cells.

2015-09-16 | E-GEOD-72367 | biostudies-arrayexpress

siRNA-mediated knockdown

Project description:siRNA-mediated knockdown

| PRJNA541912 | ENA

RNA-seq for siRNA 48h

Project description:Bulk RNA-seq were performed on E14 cells knockdown with siRNA

2023-03-08 | GSE179124 | GEO

E14 cells knockdown with siRNA or shRNA

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-10 | GSE167182 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophages Transcriptomes for Acly-E14 siRNA effects

Project description:Purpose: The purpose of this study is to detect activated or silenced genes during bone marrow derived macrophages (BMDMs) transfected with control siRNA or Acly-E14 siRNA. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. BMDMs were transfected with control siRNA or Acly-E14 siRNA. 48 hour later, they were stimulated with LPS (100ng/ml) for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between BMDMs transfected with the indicated siRNAs.

2024-11-06 | GSE240188 | GEO

ATAC-seq and H4K16ac ChIP-seq profiles of E14 ES cells treated with cycloheximide

Project description:ATAC-seq and H4K16ac ChIP-seq profiles of E14 ES cells before and after treatment with cycloheximide (CHX, 1 ug/ml) for 3 hours.

2017-07-23 | GSE98358 | GEO

Effects of knockdown of ERK5 using PROTACs and siRNA on HCT116 cells

Project description:Changes in the cellular transcriptome, proteome and phosphorylated proteome after knockdown of ERK5 protein in HCT116 cells using PROTACs and siRNA, respectively.

2022-08-23 | PXD042635 |

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