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Lorius chlorocercus

ABSTRACT: Lorius chlorocercus

PROVIDER: PRJNA703799 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR21034928_1.fastq.gz	Fastqsanger.gz
	SRR21034928_2.fastq.gz	Fastqsanger.gz

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First case report of fatal Nocardia nova infection in yellow-bibbed lory (Lorius chlorocercus) identified by multilocus sequencing.

Project description:BACKGROUND:Nocardiosis is often a multi-systemic disease in humans and other mammals. Nocardiosis in birds is uncommon. Laboratory identification of Nocardia to the species level is difficult by traditional phenotypic methods based on biochemical reactions and hydrolysis tests, and is most accurately performed by sequencing multiple gene targets. CASE PRESENTATION:We report the first case of fatal Nocardia nova infection in a yellow-bibbed lory nestling in an oceanarium diagnosed by multilocus sequencing. Necropsy examination showed effacement of normal sternal musculature with yellowish, firm aberrant material, and diffuse infiltration of the lungs with nodular, tan to yellow foci. Histologically, severe granulomatous inflammation with marked necrosis was observed in the lung, spleen and sternal musculature. Fine, sometimes Gram-positive, 0.5-1??m wide, branching and beaded filamentous organisms were visible within the lesions. They were acid-fast on Fite-Faraco stain. Tissue samples obtained from the sternum, liver, right lung and right kidney recovered Nocardia species. Sequencing of four gene loci and phylogenetic analysis of concatenated (gyrB-16S-secA1-hsp65) sequences revealed that the isolate was N. nova. CONCLUSIONS:We report the first case of N. nova infection in yellow-bibbed lorry (Lorius chlorocercus). The present case is the first one of which the species identity of the isolate was determined by multilocus sequencing. Molecular diagnosis is important for identifying the Nocardia to species level and understanding the epidemiology of nocardiosis in birds.

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The complete mitochondrial genome of yellow-bibbed lory, <i>Lorius chlorocercus</i> (Psittaciformes Psittacidae), with its phylogenetic relationship.

Project description:In this study, the complete 17,855 bp mitochondrial genome of Lorius chlorocercus was obtained using sanger sequencing. It consists of 13 protein-coding genes, 22 tRNAs, 2 ribosomal RNAs, and a control region. The overall base composition is 22.5% T, 33.18% C, 30.42% A, and13.9% G. The phylogenetic tree was constructed using neighbour-joining (NJ) method based on 19 parrot species. Phylogenetic analysis showed that L. chlorocercus was closest to Melopsittacus undulates. The complete mitogenome data would be useful for further study on the molecular evolution of L. chlorocercus.

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Comparing the chromosome binding profiles of nucleoid structuring protein H-NS in the presence or absence of conditions eliciting the depletion of transcription termination protein NusG in Salmonella

Project description: Not available

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Multigene panel test in 44 Homo sapiens patients with either digestive system neuroendocrine Neoplasm (NEN) or medullary thryoid gland carcinoma samples

Project description:The DNA isolated from 44 either frozen or FFPE Neuroendocrine Neoplasm (NEN) was analysed by NGS, to identify genes more likely to be subject to sequence variations among 523 cancer-related ones.

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