Project description:The hamstring tendon is used as autograft in surgical reconstruction of tears of the anterior cruciate ligament. The rate of re-rupture after surgery is higher in younger patients. The aim of the study was to identify molecular mechanisms that underlie the greater susceptibility of tendon from younger people to tear.
Project description:We have undertaken a screen of mouse limb tendon cells in order to identify molecular pathways involved in tendon development. Mouse limb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages.
Project description:We have undertaken a screen of mouse limb tendon cells in order to identify molecular pathways involved in tendon development. Mouse limb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages. Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were collected and dissociated with trypsin to obtain cell suspensions. Scx-positive tendon cells were isolated by FACS. RNA was extracted and Fragmented biotin-labelled cRNA samples were hybridized on Affymetrix Gene Chip Mouse Genome 430 2.0 arrays.
Project description:To identify if genes is regulated by time of day in human tendon, RNAseq analysis was performed on biopsies taken from patella tendon 12 hours apart in young healthy subjects (9 AM and 9 PM).
Project description:We have compared the gene expression profile of rat Achilles tendon-derived stem cells in post-natal tendon development. Rat tendon stem/progenitor cells (TSPCs) were isolated at different stages of post-natal development: TSPCs-1d, TSPCs-7d and TSPCs-56d.
Project description:To identify if genes is regulated by time of day in human tendinopathic tendon, RNAseq analysis was performed on biopsies taken from tendinopathic and contralateral patella tendon 12 hours apart in young tendinopathic subjects (9 AM and 9 PM).
Project description:We report the newly derived tendon like tissues from human iPSC. We depositted RNA sequencing data of human iPSC and derived MSC from human iPSC. We use these cells as materials to generae tenon-like tissues.
Project description:We have compared the gene expression profile of rat Achilles tendon-derived stem cells in post-natal tendon development. Rat tendon stem/progenitor cells (TSPCs) were isolated at different stages of post-natal development: TSPCs-1d, TSPCs-7d and TSPCs-56d. TSPCs at different post-natal development stages (1d, 7d and 56d) were isolated, cultured and used for microarray analyses at passage 2. All TSPCs in this study were of isolated from more than one individual. Total RNA was extracted and fragmented biotin-tagged cRNA was hybridized to Rat Genome 230 2.0 Array.