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ESCC KYSE150 cells: TPA treatment vs. control

ABSTRACT: ESCC KYSE150 cells: TPA treatment vs. control

PROVIDER: PRJNA703987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13764513_1.fastq.gz	Fastqsanger.gz
	SRR13764513_2.fastq.gz	Fastqsanger.gz
	SRR13764514_1.fastq.gz	Fastqsanger.gz
	SRR13764514_2.fastq.gz	Fastqsanger.gz
	SRR13764515_1.fastq.gz	Fastqsanger.gz

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ESCC KYSE150 cells: TPA treatment vs. control

Project description:Complete lncRNA and mRNA expression profile of ESCC KYSE150 cells and TPA-treatment KYSE150 cells

2024-02-01 | GSE167247 | GEO

Expression data from stably-transfected KYSE150, transfected with shlncRNA625 or shscramble

Project description:To delineate the putative biological functions for lncRNA625, We performed expression profile from stably-transfected KYSE150 transfected with shlncRNA625 or shscramble for functions of lncRNA625 in ESCC Stably-transfected KYSE150, transfected with shlncRNA625 or shscramble, were collected and lysed in TRIzol (Life technologies). Microarray experiments were performed following the Affymetrix protocol at the Shanghai Biotechnology Corporation.

2015-11-10 | E-GEOD-74707 | biostudies-arrayexpress

Gene expression of KYSE150 cells and CAFs in coculture system

Project description:KYSE150 cells and CAFs were cocultured in transwell apparatus with 0.4 μm pore size. In different groups, KYSE150 cells or CAFs plated in the lower chamber were collected for gene expression analysis using Agilent SurePrint G3 Human Gene Expression v3 (8×60K). Investigation of the critical gene participating the crosstalk between ESCC cells and cancer-associated fibroblasts (CAFs).

2025-01-25 | GSE194414 | GEO

Expression data from stably-transfected KYSE150, transfected with shlncRNA625 or shscramble

2015-11-10 | GSE74707 | GEO

Expression data in human esophageal squamous carcinoma cell line KYSE150 with LOXL2 delta 72 over-expression

Project description:We have identified a new novel spliced variant of Lysyl Oxidase-like 2 (LOXL2), termed LOXL2 delta72, in human oesophageal carcinoma cells (ESCC). To explore the biological function of this variant, the cDNA microarrays was perform to assess the genes expression induced by its over-expression in ESCC KYSE150. We used microarrays to detail the global programmed of genes expression when LOXL2 delta72 over-expressed in KYSE150 cells compared with wild-type LOXL2.

2016-03-28 | GSE53645 | GEO

Different response of WT and Ts keratinocyte to TPA treatment

2013-06-01 | GSE40382 | GEO

Different response of WT and Ts keratinocyte to TPA treatment

Project description:Ts keratinocyte has increased proliferation compare to WT keratinocyte upon TPA treatment. This result showed the different transcription regulation in Ts and WT keratinocyte upon TPA treatment. Total RNA extracted from WT or Ts keratinocytes, not treated or treated with TPA for 12 hours.

2013-06-01 | E-GEOD-40382 | biostudies-arrayexpress

Phosphorylation changes in KYSE150 cells treated with benzydamine

Project description:To explore the inhibitory mechanism of benzydamine on ESCC, we conducted phosphoproteomic analysis to comprehensively analyze the changes in the phosphorylation level of proteins of KYSE150 cells after benzydamine treatment.

2022-06-08 | PXD034404 |

The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (ChIP-seq KYSE150 cell line)

Project description:We have mapped binding sites for the histone demethylase, JMJD2C/KDM4C/GASC1, and the effect of JMJD2C depletion on H3K9me3 and H3K36me3 distributions in KYSE150 cells. The human esophageal carcinoma cell line, KYSE150, contains an amplification of the JMJD2C locus. ChIP-seq was performed using chromatin from control or JMJD2C-depleted KYSE150 cells and antibodies recognizing JMJD2C, H3K4me3, H3K9me3 or H3K36me3.

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CDCA7 promotes TGF-β-induced epithelial-mesenchymal transition via transcriptionally regulating Smad4/Smad7 in ESCC

Project description:Purpose：Cell division cycle associated 7 (CDCA7) is a copy number amplification gene that contributes to the metastasis and invasion of tumors, including esophageal squamous cell carcinoma (ESCC). This present study aimed at clarifying whether high expression of CDCA7 promotes the metastasis and invasion of ESCC cell lines and exploring the underlying mechanisms implicated in EMT of ESCC. Mehtod：We established KYSE150 cell lines in which target gene has been knocked down by shRNA and KYSE180 cell lines in which target gene has been ovexpressed by plasmid.We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells.Each cell has three repetions. Result：KEGG pathway enrichment analysis on the data of RNA-seq showed that Wnt signaling pathway and TGF-β signaling pathway which are related to EMT process were screened out. Conclusion：CDCA7 was heavily involved in EMT by regulating the expression of Smad4 and Smad7 in TGF-β signaling pathway.

2023-01-11 | GSE201735 | GEO

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