Project description:Genome wide DNA methylation profiling changes in parent and radioresistant oral cancer cells ( OCSL-P and OCSL-R ) by The Illumina Infinium WG Methylation27.
Project description:Genome wide DNA methylation profiling changes in parent and radioresistant OSCC cells (OML1 and OML1R ) by The Illumina Infinium WG Methylation27.
Project description:Genome wide DNA methylation profiling in oral cancer and oral leukoplakia patients. The cohort included 48 patients in total, with control, oral leukoplakia patients and oral cancer patients. The Infinium MethylationEPIC BeadChips 850K has been used to interrogate DNA methylation changes.
Project description:ATAC-seq identified a total 80,764 peaks in radioresistant cells (HSC-3;0Gy, 8Gy) and radiosensitive cells (KOSC-2;0Gy, 8Gy) (n=2). 1273 peaks were shown as chromatin opened regions and 839 peaks were shown as chromatin closed regions in HSC-3 8Gy compared to HSC-3 0Gy. 285 peaks were shown as chromatin opened regions and 1760 peaks were shown as chromatin closed regions in KOSC-2 8Gy compared to KOSC-2 0Gy. 3804 peaks were shown as chromatin opened regions and 1929 peaks were shown as chromatin closed regions in KOSC-2 0Gy compared to HSC-3 0Gy. By hierarchical clustering analysis and heatmap, the difference of chromatin accessibility in KOSC-2 was lower than HSC-3.
Project description:We established radioresistant cell sublines, named as BxPC3/RR1 and BxPC3/RR2 which were derived from parental human pancreatic cancer cell line BxPC3. The miRNA expression profiles of the radioresistant pancreatic cancer cells were then compared with their parental cells. Three samples were analysed. miRNAs that were differentially expressed (increased or decreased in expression by M-bM-^IM-%1.5-fold) between the radioresistant and parental cells were identified.
Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in DU145 ALDH+ prostate cancer progenitors and DU145-RR radioresistant cells. Non-sorted, ALDH-negative, and ALDH-positive populations were analyzed, both for parental DU145 cell line and for its radioresistant sub-line. Three independent experiments were performed