Project description:SPARC binding proteins detected by mass spectrometer. Cell lysate were collected from HepG2 cells by non-denaturing lysis buffer. Total cell proteins were immnuoprecipitated by SPARC specific antibody from R&D company. SPARC and binding proteins were loaded on SDS-PAGE gel and ran for 30 minutes. The gel was stained with Coomassie Blue and SPARC-binding proteins in gel were cut and send for mass analysis for the detection of the specific proteins.
Project description:We identified that Sparc gene expression is upregulated in corneal epithelial cells in a mouse model of dry eye disease involving lacrimal gland excision. Therefore, in this experiment we assess the effect of SPARC treatment on the transcriptome of human corneal epithelial cells.
Project description:The aim of this study was evaluate the transcriptome changes in the comparison between triple negative tumors with increased SPARC expression and triple negative tumors with decreased SPARC expression according to Nagai et al., 2011 (Breast Cancer Res Treat (2011) 126:1–14) The results generated could be of particular interest to better define the prognostic impact of SPARC expression in triple negative breast tumors
Project description:A time course of the effect of overexpressing GPI-PLD on global gene expression in HepG2 cells was compared to control virus. Keywords: Expression profiling
Project description:SPARC-deficient mice have been shown to exhibit impaired glucose tolerance and insulin secretion, but the underlying mechanism remains unknown. We used microarrays to detail the global programme of gene expression and identified distinct classes of up-regulated and down-regulated genes changed by overexpressing SPARC.