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TT-seq and RNA-seq in TX1072 XXΔXic and XO mESCs during differentiation

ABSTRACT: TT-seq and RNA-seq in TX1072 XXΔXic and XO mESCs during differentiation

PROVIDER: PRJNA704378 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13771276_1.fastq.gz	Fastqsanger.gz
	SRR13771276_2.fastq.gz	Fastqsanger.gz
	SRR13771277_1.fastq.gz	Fastqsanger.gz
	SRR13771277_2.fastq.gz	Fastqsanger.gz
	SRR13771278_1.fastq.gz	Fastqsanger.gz

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RNA-seq in TX1072 XO mESCs during differentiation

Project description:RNA-seq in TX1072 XO mESCs during differentiation

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ATAC-seq in TX1072 XXΔXic and XO mESCs during differentiation

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TT-seq and RNA-seq in TX1072 XXΔXic and XO mESCs during differentiation

Project description:Developmental genes are controlled by complex cis-regulatory landscapes that integrate multiple signals to ensure the correct spatio-temporal expression pattern. To investigate the underlying regulatory principles, we use the Xist locus as a model, which encodes the master regulator of X-chromosome inactivation. Xist is upregulated at the primed pluripotent state in a female-specific manner, thus integrating developmental cues and X-dosage information. It remains poorly understood how these signals are decoded by the ~800kb genomic region that controls Xist. While a series of repressive cis-regulatory elements have been identified, the distal enhancers that activate Xist transcription remain largely unknown. Here we use TT-seq and RNA-seq to profile nascent and processed RNA transcription within the X inactivation center at the onset of random X-chromosome inactivation using an endogenous cell model of the inactive (TX 1072 XXΔXic) and active (TX 1072 XO) X chromosome.

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RNA-seq in TX1072 XO mESCs during differentiation

Project description:As the master regulator of X-chromosome inactivation (XCI), the Xist RNA is expressed nearly ubiquitously in female mice. Xist is only absent in the germ line and at the pluripotent state. Xist is generally assumed to be expressed “by default” in females, while being actively repressed in the few tissues where it is silent. Whether activating mechanisms also contribute remained largely unknown. Through a pooled CRISPR screen we identify the GATA family of transcription factors as potent direct activators of Xist. We describe a GATA-responsive regulatory element (RE79), located ~100 kb upstream of the Xist promoter. In cell lines derived from the two extraembryonic lineages, XEN and TS cells, where imprinted Xist expression is maintained, RE79 is bound by different sets of GATA factors expressed in those tissues. Here we use RNA-seq on differentiating XO mouse embryonic stem cells as a reference for gene expression during early differentiation.

2022-01-26 | GSE194016 | GEO

ATAC-seq in TX1072 XXΔXic and XO mESCs during differentiation

Project description:Developmental genes are controlled by complex cis-regulatory landscapes that integrate multiple signals to ensure the correct spatio-temporal expression pattern. To investigate the underlying regulatory principles, we use the Xist locus as a model, which encodes the master regulator of X-chromosome inactivation. Xist is upregulated at the primed pluripotent state in a female-specific manner, thus integrating developmental cues and X-dosage information. It remains poorly understood how these signals are decoded by the ~800kb genomic region that controls Xist. While a series of repressive cis-regulatory elements have been identified, the distal enhancers that activate Xist transcription remain largely unknown. Here we use ATAC-seq to profile DNA accessibility within the X inactivation center at the onset of random X-chromosome inactivation using an endogenous cell model of the inactive (TX 1072 XXΔXic) and active (TX 1072 XO) X chromosome.

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Capture Hi-C in TX1072 XXΔXic and XO mESCs in the naive pluripotent state and during differentiation

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PolyA-tail enriched RNA-seq in differentiating TX1072 XX mESCs

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STARR-seq in 1.8 XX and XO mESCs during differentiation

Project description:STARR-seq in 1.8 XX and XO mESCs during differentiation

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CUT&Tag on H3K4me3, H3K27ac, H3K4me1, H3K9me3, H3K27me3, H3K36me3 and H2AK119ub in TX1072 XXΔXic and XO mESCs during differentiation

Project description:CUT&Tag on H3K4me3, H3K27ac, H3K4me1, H3K9me3, H3K27me3, H3K36me3 and H2AK119ub in TX1072 XXΔXic and XO mESCs during differentiation

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