Project description:The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on monosaccharides in an effort to determine whether carbon catabolite repression was active. Chemostat studies and global transcriptome analysis were used to accomplish this goal.
Project description:The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and the effect of pentose and hexose sugars on gene expression Chemostat studies and global transcriptome analysis were used to accomplish this goal.
Project description:A proteomic analysis of a redox active membrane fraction (doublet at >200 kDa) of Mariprofundus ferrooxydans, a chemolithoautotrophic, neutrophilic, iron-oxidizing Zetaproteobacterium.
Project description:Proteomic analysis was performed on protein extracts of M. ferrooxydans by following two methodologies: osmotic shock and alkalyne lysis (extraction with NaOH).
Project description:A proteomic profile of the chemolithoautotrophic, neutrophilic, iron-oxidizing Mariprofundus ferrooxydans was obtained in duplicates and analyzed via LC-MS/MS. Additionally, a proteomic analysis of the membrane fraction is included.