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Analysis of differentially expressed miRNA in cisplatin-resistant FaDu cell

ABSTRACT: Analysis of differentially expressed miRNA in cisplatin-resistant FaDu cell

PROVIDER: PRJNA704564 | ENA |

REPOSITORIES: ENA

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Analysis of differentially expressed miRNA in cisplatin-resistant FaDu cell

Project description:Cisplatin is a common chemotherapeutic drug for hypopharyngeal cancer. But cisplatin-resistance of hypopharyngeal cancer is rarely explored. We cultured hypopharyngeal cancer cell (FaDu) and induced its cisplatin-resistant cell (FaDu/DDP4). The resistance index (RI) of FaDu/DDP4 was 2.828. Then we tested the differentially expressed genes (DEGs) between FaDu and FaDu/DDP4. DEGs contain 2388 lncRNAs, 1932 circRNAs, 745 mRNAs and 202 miRNAs. We used Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed the DEGs. The differentially expressed 745 mRNAs were classified into 3 domains and 47 secondary GO terms. In KEGG pathway enrichment, “TNF signaling pathway”, “IL-17 signaling pathway” and “JAK-STAT signaling pathway” have greater enrich factors. And we drew the ceRNA networks of DEGs. 52 lncRNAs, 148 circRNAs, 155 mRNAs and 18 miRNAs were selected to draw the network. We noticed several potential targets (as miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1). At last, we chose 8 miRNAs and 6 mRNAs for qRT-PCR to verify our microarray. In them, miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1 might be potential genes inducing resistance.

2021-06-02 | GSE167398 | GEO

Analysis of differentially expressed lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

2021-06-02 | GSE167396 | GEO

Analysis of differentially expressed miRNA, lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

Project description:Analysis of differentially expressed miRNA, lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

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Analysis of differentially expressed lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

Project description:Analysis of differentially expressed lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

| PRJNA704562 | ENA

Basal mRNA expression levels of single-cell derived FaDu clones with cisplatin-sensitive / resistant phenotype

Project description:The extent of intratumoral heterogeneity, the subclonal structures and the mechanisms of treatment-induced clonal selection by cisplatin was investigated in the squamous cell carcinoma cell line model FaDu. We picked 96 single cell-derived clones from the cisplatin-sensitive parental FaDu cell line. After expansion as separate cultures, these clones were tested for their sensitivity to CDDP. By this approach, we isolated individual cell clones that were primarily resistant (clones 5 & 78) and others that showed high sensitivity to CDDP (clones 46 & 54). Basal mRNA expression levels associated with CDDP sensitivity / resistance were determined in two independent microarray analyses.

2017-12-04 | E-MTAB-6022 | biostudies-arrayexpress

Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines Panc-1, FaDu and PC3

Project description:To identify differentially expressed genes by anti cancer treatments (microRNAs or siRNAs) in human cancer, several cell lines (pancreatic cancer, hypopharyngeal squamous cell carcinoma and prostate cancer) were subjected to Agilent whole genome microarrays. Human cell lines (Panc-1, FaDu and PC3) were treated with miRNAs (miR99a-5p, miR-99a-3p, miR-100-3p, miR-150-5p and miR-150-3p), siRNAs (si-FOXQ1).

2016-08-16 | E-GEOD-85614 | biostudies-arrayexpress

Analysis of differentially expressed miRNA, lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-02 | GSE167399 | GEO

Identification of differentially expressed genes in cisplatin-resistant and parental esophageal squamous cell carcinoma cells

Project description:Identification of differentially expressed genes in cisplatin-resistant and parental esophageal squamous cell carcinoma cells

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Analysis of ceRNA network of differentially expressed genes in FaDu cell line and a cisplatin-resistant line derived from it.

Project description:BackgroundHypopharyngeal cancer accounts for 2% in head and neck cancers and has a poor prognosis. Cisplatin is a widely used chemotherapeutic drug in kinds of carcinomas, concluding hypopharyngeal cancer. However, the resistance of cisplatin appeared in recent years. Cisplatin-resistance has been partly explored before, but rarely in hypopharyngeal cancer.MethodsWe cultured the hypopharyngeal cancer cell (FaDu) and induced its cisplatin-resistant cell (FaDu/DDP4). Then we tested the differentially expressed genes (DEGs) between FaDu and FaDu/DDP4. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted on the DEGs, and we drew the ceRNA networks of DEGs. Finally, we chose eight miRNAs and six mRNAs for qRT-PCR to verify our microarray.ResultsWe induced cisplatin-resistant FaDu/DDP4 and proved its chemoresistance. The resistance index (RI) of FaDu/DDP4 was 2.828. DEGs contain 2,388 lncRNAs, 1,932 circRNAs, 745 mRNAs and 202 miRNAs. These 745 mRNAs were classified into three domains and 47 secondary GO terms. In KEGG pathway enrichment, the "TNF signaling pathway", "IL-17 signaling pathway" and "JAK-STAT signaling pathway" were potentially significant signaling pathways. Then, 52 lncRNAs, 148 circRNAs, 155 mRNAs and 18 miRNAs were selected to draw the network. We noticed several potential targets (as miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1). At last, the eight miRNAs and six mRNAs that are critical RNAs in ceRNA network were verified by qRT-PCR.ConclusionThe microarray helped to find DEGs in cisplatin-resistant hypopharyngeal cancer. TNF, IL-17 and JAK-STAT signaling pathways might be more significant for cisplatin-resistance. MiR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1 might be potential genes inducing resistance.

| S-EPMC8255068 | biostudies-literature

Differentially expressed miRNA in recurrent implantation failure (RIF)

Project description:Differentially expressed miRNA in recurrent implantation failure (RIF)

| PRJNA496396 | ENA