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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

ABSTRACT: Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

PROVIDER: PRJNA704636 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13777347.fastq.gz	Fastqsanger.gz
	SRR13777348.fastq.gz	Fastqsanger.gz
	SRR13777349.fastq.gz	Fastqsanger.gz
	SRR13777352.fastq.gz	Fastqsanger.gz
	SRR13777353.fastq.gz	Fastqsanger.gz

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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

Project description:Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

Project description:The early limb bud consists of mesenchymal progenitors (limb progenitors) derived from the lateral plate mesoderm (LPM) that produce most of the tissues of the mature limb bud. The LPM also gives rise to the mesodermal components of the trunk, flank and neck. However, the mesenchymal cells generated at these other axial levels cannot produce the variety of cell types found in the limb bud, nor can they be directed to form a patterned appendage-like structure, even when placed in the context of the signals responsible for organizing the limb bud. Here, by taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28) normally expressed in the early limb bud, that are capable of imparting limb progenitor-like properties to non-limb fibroblasts. Cells reprogrammed by these factors show similar gene expression profiles, and can differentiate into similar cell types, as endogenous limb progenitors. The further addition of Lin41 potentiates proliferation of the reprogrammed cells while suppressing differentiation. These results suggest that these same four key factors may play pivotal roles in the specification of endogenous limb progenitors.

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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

Project description:Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

| PRJNA704626 | ENA

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-15 | GSE167423 | GEO

Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

Project description:Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

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Direct Conversion of Human Fibroblasts into Neuronal Restricted Progenitors

Project description:Neuronal restricted progenitors (NRPs) represent a type of transitional intermediate cells that lie between multipotent neural progenitors (NPs) and terminal differentiated neurons during neurogenesis. These NRPs have the ability to self-renew and differentiate into neurons, but not into glial cells, which is considered as an advantage for cellular therapy of human neurodegenerative diseases. However, difficulty in the extraction of highly purified NPRs from normal nervous tissue prevents further studies and applications. In this study, we reported conversion of human fetal dermal fibroblasts into human induced neuronal restricted progenitors (hiNRPs) in seven days by using just three defined factors: Sox2, c-Myc, and either Brn2 or Brn4. The hiNRPs exhibited distinct neuronal characteristics, including cell morphology, multiple neuronal markers expression, self-renewal capacity, and genome-wide transcriptional profile. Moreover, hiNRPs were able to differentiate into various terminal neurons with functional membrane properties, but not glial cells. Direct generation of hiNRPs from somatic cells will provide a new source of cells for cellular replacement therapy of human neurodegenerative diseases. This is a general expression microarray design (NimbleGen platform). It includes 5 samples.

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Direct reprogramming of mouse fibroblasts to neural progenitors.

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Project description:Blastema formation is a hallmark of limb regeneration that requires proliferation and migration of progenitors derived from many tissues to the amputation plane. To better understand the genetic programs that initiate limb regeneration, we reasoned that blastemal progenitors would be among early proliferating cells in the stump following amputation. Here we separately profiled dividing and non-dividing stump tissues, as well as the wound epidermis, during early axolotl limb regeneration to examine transcriptional programs of blastemal progenitors. We provide a description of the changes in gene expression specific to early dividing cells at the site of amputation, inclusive of progenitors for the regenerating limb. This work collectively demonstrates differential suppression/activation of core developmental signaling pathways in subsets of the early regenerating limb and further suggests that interleukin-8 (il-8) signaling is important for regeneration.

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