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Differential gene expression analysis between Wild type(WT) and pbl19/pPBL19C3A-GFP-HA transgenic plants

ABSTRACT: Differential gene expression analysis between Wild type(WT) and pbl19/pPBL19C3A-GFP-HA transgenic plants

PROVIDER: PRJNA705200 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13797240.fastq.gz	Fastqsanger.gz
	SRR13797241.fastq.gz	Fastqsanger.gz

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Differential gene expression analysis of pbl19/pPBL19C3A-GFP-HA transgenic plants

Project description:Purpose: RNA-seq analysis of the pbl19/pPBL19:PBL19C3A-GFP-HA, sid2pbl19/pPBL19:PBL19C3A-GFP-HA and eds1pbl19/pPBL19:PBL19C3A-GFP-HA plants and identified a large number of differentially expressed genes in these plants relative to wild-type plants. Methods: For RNA-seq analyses, a total amount of 1 mg RNA per sample was used for RNA-seq library construction. Results: RNA-seq analysis of wild type(WT), pbl19/pPBL19:PBL19C3A-GFP-HA, sid2pbl19/pPBL19:PBL19C3A-GFP-HA and eds1pbl19/pPBL19:PBL19C3A-GFP-HA at 28 DAG.Numbers of differentially expressed genes in pbl19/pPBL19::PBL19C3A-GFP-HA plants compared to WT.1981 differentially expressed genes were constantly up-regulated in pbl19/pPBL19::PBL19C3A-GFP-HA plants compared to WT Conclusions: Receptor-like cytoplasmic kinase PBL19 exhibited the highest transcriptional upregulation among the forty-six genes of the RLCK VII subfamily in pbl19/pPBL19C3A-GFP-HA and sid2 pbl19/pPBL19C3A-GFP-HA transgenic plants.eds1 pbl19/pPBL19:PBL19C3A-GFP-HA plants were fully restored to a wild-type(WT) appearance

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Differential gene expression analysis between Wild type(WT) and pbl19/pPBL19C3A-GFP-HA transgenic plants

Project description:Purpose: The goals of this study is compared the differential gene expression between WT and pbl19/pPBL19C3A-GFP-HA transgenic plants Methods: For RNA-seq analyses, a total amount of 1 mg RNA per sample was used for RNA-seq library construction. RNA-seq on the BGISAQ-500 with 150 bp paired-end reads and data analysis were carried out by the Beijing Genomics institution Results: RNA-seq analysis of Col-0 and pbl19/pPBL19::PBL19C3A at 28DAG.Numbers of differentially expressed genes in pbl19/pPBL19::PBL19C3A plants compared to WT.1757 differentially expressed genes were constantly up-regulated in pbl19/pPBL19::PBL19C3A plants compared to WT Conclusions: Receptor-like cytoplasmic kinase PBL19 exhibited the highest transcriptional upregulation among the forty-six genes of the RLCK VII subfamily. 699 differentially expressed genes was down-regulated in pbl19/pPBL19::PBL19C3A plants compared to WT.

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