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ABSTRACT: Comparative developmental transcriptomics of Myxococcus xanthus and Myxococcus stipitatus

PROVIDER: PRJNA705220 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13798945.fastq.gz	Fastqsanger.gz
	SRR13798946.fastq.gz	Fastqsanger.gz
	SRR13798947.fastq.gz	Fastqsanger.gz
	SRR13798948.fastq.gz	Fastqsanger.gz
	SRR13798949.fastq.gz	Fastqsanger.gz

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Project description:Comparative developmental transcriptomics of Myxococcus xanthus and Myxococcus stipitatus

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Project description:Phosphate regulation is complex in the developmental prokaryote Myxococcus xanthus, and requires at least three previously characterized two-component systems (TCS), designated PhoR1-PhoP1, PhoR2-PhoP2, and PhoR3-PhoP3. We report here the identification and characterization of a member of a fourth TCS, designated PhoP4, which shows high sequence similarity to the other three M. xanthus PhoP response regulators. PhoP4 is an orphan response regulator that was identified by yeast 2-hybrid screen as a possible interacting partner of PhoR2. phoP4 insertion mutation and in-frame deletion strains were constructed and assessed for developmental and phosphatase phenotypes, and then compared with the phenotypes previously reported for a _phoR2-phoP2 strain. The data indicate that the phoP4 mutations caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80-90% under phosphate-limiting conditions. These phenotypes are stronger than those observed for mutations in the PhoR2-PhoP2 system, thereby suggesting a high degree of complexity vis-à-vis the interactions amongst the Pho signal transduction pathways in M. xanthus. Keywords: developmental analysis

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Project description:TraA/B mediated transcriptomics in Myxococcus xanthus

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