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Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction

ABSTRACT: Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction

PROVIDER: PRJNA705678 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13815900_1.fastq.gz	Fastqsanger.gz
	SRR13815900_2.fastq.gz	Fastqsanger.gz
	SRR13815901_1.fastq.gz	Fastqsanger.gz
	SRR13815901_2.fastq.gz	Fastqsanger.gz
	SRR13815902_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2020RNAseqData]

Project description:S. coelicolor spore stocks were diluted to 2x108 cfu/mL and 0.5ul was spotted onto a 60x15mm petri dish with 4ml ISP2 agar, resulting in agar approximately 2 mm thick. For interactions, 0.5 ul of an Amycolatopsis sp. AA4 stock (8x108 cfu/mL) was spotted 0.75 cm away from the S. coelicolor spot. Interactions were grown for 4 days at 30°C, then imaged or harvested for RNA isolation. Biomass was collected after 4 days of growth at 30°C using a cell scraper. For the patches growing alone, the entire patch was collected and one sample consists of three whole patches combined. For the patches in interactions, only half of the patch, the side closest to the initiator strain, was collected and one sample consists of five half patches. Precaution was taken to not scrape up any of the interacting strain.

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Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2017RNAseqData]

2021-09-08 | GSE167964 | GEO

Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2017RNAseqData]

Project description:Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2017RNAseqData]

| PRJNA705680 | ENA

Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2020RNAseqData]

Project description:Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction [2020RNAseqData]

| PRJNA705681 | ENA

Transcriptomic analysis of Streptomyces coelicolor Cvn8 mutants on ISP2 agar in isolation or in an interaction

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcription profiles of che and flgM mutants of Typhimurium 14028 at 3h on agar plates

Project description:Transcription profiles of Typhimurium 14028 wt versus 14028 cheA, cheB, cheZ, flgM and flgM/cheY mutants at 3h on agar plates Keywords: other

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Project description:Global gene expression patterns of S. coelicolor MT1151 and MT1153 (two independent MT1110 hspR disruption mutants ) cultures compared to MT1152 and MT1154 (two independent isolates of hspR+ integrants), grown on SMMS agar medium for 36 h .

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Project description:S. coelicolor M145 and S. coelicolor ∆argR were grown in MG medium and samples from 3 biological replicates were taken at 32, 42, 49, 56 and 66 hours.

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