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ABSTRACT: Transcriptomic analysis of Listeria monocytogenes in response to bile under aerobic and anaerobic conditions

PROVIDER: PRJNA706425 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13849950_1.fastq.gz	Fastqsanger.gz
	SRR13849950_2.fastq.gz	Fastqsanger.gz
	SRR13849951_1.fastq.gz	Fastqsanger.gz
	SRR13849951_2.fastq.gz	Fastqsanger.gz
	SRR13849952_1.fastq.gz	Fastqsanger.gz

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The effect of oxygen on bile resistance in L. monocytogenes

Project description:Listeria monocytogenes is a gram-positive, facultative anaerobe food-borne pathogen that is the causative agent of listeriosis. Upon ingestion, L. monocytogenes is subjected to a variety of non-specific host defenses such as bile. The main component of bile is bile salts which is known to be bactericidal through inducing DNA damage, oxidative damage, membrane instability, and protein misfolding. Previous studies have focused mainly on aerobic conditions; however the human GI tract is an environment ranging from microaerophilic to anaerobic. The bile salt hydrolase, an enzyme known to reduce toxicity of bile through hydrolysis, has been shown to have an increase in activity under anaerobic conditions. Therefore, the purpose of this study was to determine the effect of oxygen on bile resistance and determine the bile specific proteome that is responsible for this. To do this, we performed survival assays on virulent strains: F2365, 10403S, EGDe and avirulent strain, HCC23. Results showed an increase in viability for all virulent strains when exposed to porcine bile under anaerobic conditions. Interestingly, an increase in resistance was seen in HCC23 only under aerobic conditions. We then used a total proteomic analysis approach to compare the bile specific proteome under both aerobic and anaerobic conditions. Results showed many difference between all strains including an increase in abundance of proteins associated with stress responses, repair, cell morphology, and cell division under anaerobic conditions. Response to bile salt stress showed to be strain dependent. This study not only identified proteins responsible for L. monocytogenes bile resistance but also differerences between aerobic and anaerobic conditions thus suggesting that oxygen availability is not only a stressor but in some way providing assistance to overcoming bile salts.

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Project description:Transcriptomic analysis of Listeria monocytogenes in response to bile under aerobic conditions at pH 7.5

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Project description:Transcriptomic analysis of Listeria monocytogenes in response to bile under aerobic conditions at pH 5.5

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Project description:Transcriptomic analysis of Listeria monocytogenes in response to bile under anaerobic conditions at pH 5.5

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Project description:These studies were designed to examine the acute Listeria monocytogenes transcriptional response to mammalian (porcine) bile. Triplicate WT Listeria monocytogenes (strain 10403S) were grown to mid-log in BHI at 37 Â°C. Samples were divided, and either treated or not treated by addition of porcine bile (Sigma, to 1% final) for 30 minutes.

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