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Desiccation-driven senescence in the resurrection plant Xerophyta schlechteri

ABSTRACT: Desiccation-driven senescence in the resurrection plant Xerophyta schlechteri

PROVIDER: PRJNA706599 | ENA |

REPOSITORIES: ENA

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Desiccation-driven senescence in the resurrection plant Xerophyta schlechteri

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The transcriptome response of leaves of the resurrection plant Xerophyta humilis to desiccation

Project description:Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in Xerophyta humilis leaves at 6 relative water contents (RWC) (100%, 80%, 60%, 40%, 20% and 5%RWC respectively). 3105 cDNA clones (corresponding to 1709 unique cDNAs) were randomly selected from X. humilis cDNA libraries (Leaf Dehydration (LD), Leaf Rehydration (LR), Root Dehydration (RD) and Root Rehydration (RR)), amplified by PCR, and printed on each glass slide multiple times (ranging from 4 to 12X), such that each printed block contained a random unbiased mixture of cDNAs from the 4 different cDNA libraries. Total RNA extracted from each X. humilis leaf sample was linearly amplified, labelled with Cy3, and simultaneously hybridized with Cy5 labelled common reference RNA, to the printed cDNA arrays. The reference RNA comprised equal amounts of total RNA pooled from 6 different RWC experimental samples. Data was recorded for five biological replicates for each of the 6 RWC stages being investigated.

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Global transcriptome analysis reveals major roles for protein quality control, autophagy, and reactive oxygen species scavenging during the acquisition of desiccation tolerance in the resurrection plant Boea hygrometrica

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Project description:Vegetative desiccation tolerance, the ability to survive loss of ~ 95% relative water content (RWC) is rare in angiosperms, these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the res-urrection plant Xerophyta schlechteri in response to desiccation. A targeted metabolomics approach validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified. An early response to drying, during which the leaf tissues decline from full turgor to a relative water content (RWC) of ~ 80 - 70%, a mid-response in which RWC declines to 40% and a late response where tissues decline to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tol-erance of this species.

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