Project description:The methylotrophic, thermotolerant yeast Ogataea parapolymorpha (formerly Hansenula polymorpha) is an industrially relevant production host and exhibits a respiratory metabolism in the presence of oxygen. It possesses a branched respiratory chain with multiple entry points for NADH-derived electrons that differ in complexity and degree of energy conservation: proton-translocating respiratory Complex I and three putative alternative NADH dehydrogenases. To investigate the physiological importance of Complex I, wild type O. parapolymorpha and a Complex I-disrupted mutant were cultured in glucose-grown bioreactor experiments in batch, chemostat and retentostat cultivations which allowed quantitative characterization of the strains over a wide range of growth rates in the presence and absence of excess substrate.
Project description:Transcriptional profiling of hac1 deletion mutant compared to wild type (control). Wild type vs. hac1 deletion mutant of Ogataea angusta DL1 strain in exponential growth. Two biological replicates, with dye-swaps.
Project description:This is genome-scale metabolic model of Komagataella pastoris as the representative yeast species for the clade Pichiaceae. This model was generated through homology search using a fungal pan-GEM largely based on Yeast8 for Saccharomyces cerevisiae, in addition to manual curation. This model has been produced by the Yeast-Species-GEMs project from Sysbio (www.sysbio.se). This is model version 1.0.0 accompanying the publication (DOI: 10.15252/msb.202110427), currently hosted on BioModels Database and identified by MODEL2109130009. Further curations of this model will be tracked in the GitHub repository: https://github.com/SysBioChalmers/Yeast-Species-GEMs Models for species of the same clade includes: Ambrosiozyma kashinagacola; Ambrosiozyma monospora; Brettanomyces anomalus; Candida arabinofermentans; Candida boidinii; Candida sorboxylosa; Candida succiphila; Brettanomyces bruxellensis; Komagataella pastoris; Kuraishia capsulata; Ogataea methanolica; Ogataea parapolymorpha; Ogataea polymorpha; Pichia membranifaciens; Ogataea henricii; Ambrosiozyma ambrosiae; Citeromyces matritensis; Ambrosiozyma vanderkliftii; Brettanomyces custersianus; Komagataella populi; Saturnispora hagleri; Saturnispora mendoncae; Saturnispora saitoi; Saturnispora serradocipensis; Saturnispora silvae; Saturnispora zaruensis; Pichia occidentalis; Pichia norvegensis; Pichia nakasei; Pichia kudriavzevii; Pichia heedii; Pichia exigua; Martiniozyma abiesophila; Ogataea nitratoaversa; Ogataea populialbae; Ogataea zsoltii; Ogataea trehalophila; Ogataea trehaloabstinens; Ogataea ramenticola; Ogataea pini; Ogataea pilisensis; Ogataea philodendri; Ogataea glucozyma; Ogataea kodamae; Ogataea methylivora; Ogataea minuta; Ogataea naganishii; Ogataea nonfermentans; Kuraishia ogatae; Kuraishia molischiana; Komagataella pseudopastoris; Ambrosiozyma oregonensis; Ambrosiozyma philentoma; Citeromyces hawaiiensis; Citeromyces siamensis; Ambrosiozyma maleeae; Ambrosiozyma pseudovanderkliftii; Pichia terricola; Saturnispora dispora; Kregervanrija delftensis; Kregervanrija fluxuum. These models are available in the zip file. To cite BioModels, please use: V Chelliah et al; BioModels: ten-year anniversary. Nucleic Acids Res 2015; 43 (D1): D542-D548. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to MIT License for more information.
Project description:We analyze the transcriptome of Ogataea parapolymorpha wild-type and met4 mutant under sulfur limited condition to investigate the function of Met4 protein in this yeast in the absence of any sulfur source. By sequencing the mRNAs of the wild-type and mutant grown in sulfur-limited B minimal medium with or without cysteine as a sole sulfur source, we find the role of OpMet4p as a master regulator for cell homeostasis under sulfur limitation.