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Digitalis purpurea

ABSTRACT: Herbaceous plant with significant ornamental and medicinal value.

PROVIDER: PRJNA70679 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR128113_1.fastq.gz	Fastqsanger.gz
	SRR128113_2.fastq.gz	Fastqsanger.gz
	SRR128114_1.fastq.gz	Fastqsanger.gz
	SRR128114_2.fastq.gz	Fastqsanger.gz
	SRR128115_1.fastq.gz	Fastqsanger.gz

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Digitalis purpurea

Project description:Digitalis purpurea RNA-Seq - Medicinal Plants Consortium

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Project description:The transcriptome sequencing of Digitalis purpurea leaves

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Digitalis purpurea

Project description:Digitalis purpurea Raw sequence reads

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Comprehensive analysis of alternative splicing in Digitalis purpurea by strand-specific RNA-Seq.

Project description:Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific sequencing information, the post-transcriptional mechanism of gene regulation in D. purpurea thus far remains unknown. In this study, a strand-specific RNA-Seq library was constructed and sequenced using Illumina HiSeq platforms to characterize the transcriptome of D. purpurea with a focus on alternative splicing (AS) events and the effect of AS on protein domains. De novo RNA-Seq assembly resulted in 48,475 genes. Based on the assembled transcripts, we reported a list of 3,265 AS genes, including 5,408 AS events in D. purpurea. Interestingly, both glycosyltransferases and monooxygenase, which were involved in the biosynthesis of cardiac glycosides, are regulated by AS. A total of 2,422 AS events occurred in coding regions, and 959 AS events were located in the regions of 882 unique protein domains, which could affect protein function. This D. purpurea transcriptome study substantially increased the expressed sequence resource and presented a better understanding of post-transcriptional regulation to further facilitate the medicinal applications of D. purpurea for human health.

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Chlorophytum borivilianum

Project description:Annual herb of medicinal value

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Transcriptome analysis of marigold color

Project description:Marigold (Tagetes erecta L.) is an important ornamental plant with a wide variety of colors. Despite its economic value, there are few biochemical and molecular basic studies of flower color in marigold. To study the mechanism behind its color formation, metabolomics analysis and de novo cDNA sequencing was performed on marigold inbred line ‘V-01’ and its petal color mutant ‘V-01M’, in four flower developmental stages.

2019-10-14 | GSE138728 | GEO

Project description:The complete chloroplast genome of Agapanthus praecox (Agapanthaceae), an ornamental and medicinal plant

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Fingerprinting the Asterid species using subtracted diversity array reveals novel species-specific sequences.

Project description:Asterids is one of the major plant clades comprising of many commercially important medicinal species. One of the major concerns in medicinal plant industry is adulteration/contamination resulting from misidentification of herbal plants. This study reports the construction and validation of a microarray capable of fingerprinting medicinally important species from the Asterids clade.

2014-07-31 | GSE31242 | GEO

Enhanced growth and cardenolides production in Digitalis purpurea under the influence of different LED exposures in the plant factory.

Project description:In this report, we have investigated the influence of different light qualities on Digitalis purpurea under a controlled environment. For this purpose, red (R), blue (B), fluorescent lamp (FL, control), along with combined red and blue (R:B) LEDs were used. Interestingly, the plant growth parameters such as number of leaf, longest root, width of leaf, width of stomata, width of trichome, leaf area, leaf or root fresh weight (FW), weight (DW) as well as length of trichome were maximum under R:B (8:2), and significantly larger than control plants. The stomatal conductance or anthocyanin was maximum under B LED than those under FL, however the photosynthesis rate was greater under FL. RuBisCO activity was maximum under R:B (1:1) LEDs while the quantity of the UV absorbing substances was highest under R LED than under FL. The maximum amount of cardenolides were obtained from leaf tissue under R:B (2:8) LED than those under FL. The R:B LEDs light was suitable for Digitalis plant growth, development, micro- and macro-elements, as well as cardenolides accumulation in the plant factory system. The adaptation of the growth strategy developed in this study would be useful for the production of optimized secondary metabolites in Digitalis spp.

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Project description:Medicinal Plant

| PRJNA505513 | ENA