Project description:Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, immune responses, and communicating with the environment. Gut microbiota can be affected by some external factors such as foods, temperature, and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops all over the world. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed the S. frugiperda with the antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb the gut microbiota, and further examined the effect of dysbiosis in gut microbiota on the gene expression of S. frugiperda by RNA sequencing. We found the composition and diversity of the gut bacterial community were changed in S. frugiperda after antibiotics treatmen, and the expression of genes related to energy and metabolic process were affected after antibiotics exposure in S. frugiperda. Our work will help understand the role of gut microbiota in insects.
Project description:Purpose: We analyzed the 3rd-instar Spodoptera frugiperda response after SfAV-1a infection. Specifically, we targeted three gene types in the infected host namely, mitochondrial, cytoskeleton and innate immunity genes.
Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series