Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome changes during inducable gametocytogenesis in Plasmodium berghei parasites

ABSTRACT: Transcriptome changes during inducable gametocytogenesis in Plasmodium berghei parasites

PROVIDER: PRJNA714084 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13953450_1.fastq.gz	Fastqsanger.gz
	SRR13953450_2.fastq.gz	Fastqsanger.gz
	SRR13953451_1.fastq.gz	Fastqsanger.gz
	SRR13953451_2.fastq.gz	Fastqsanger.gz
	SRR13953452_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

Transcriptome changes during inducible gametocytogenesis in Plasmodium berghei parasites

Project description:During the malaria infection, Plasmodium parasites invade the host’s red blood cells where they can differentiate into two different life forms. The majority will replicate asexually and infect new erythrocytes. A small percentage, however, will transform into gametocytes – a specialized sexual stage able to survive and develop when taken up by Anopheles mosquito. As the gametocytes ensure the parasite’s transmission to a new host, their generation is an attractive target for new antimalarial interventions. The molecular mechanisms controlling gametocytogenesis, however, remain largely unknown due to the technical challenges: the early gametocytes are morphologically indistinguishable from asexual parasites and present in very low numbers during the infection. Recently, AP2-G - a transcription factor from an apicomplexa-specific apiAP2 family – was described as indispensable for gametocyte commitment in both human malaria parasite Plasmodium falciparum and rodent malaria model Plasmodium berghei. Therefore, we have decided to test whether the overexpression of this factor alone could increase gametocyte production and enable the investigation of uncharacterised, earliest stages of gametocyte development. To this end, we have engineered PBGAMi - a Plasmodium berghei line, in which all parasites were ap2-g deficient by default but able to overexpress it when induced with rapamycin. While the control parasites (PBGAMi R-), as expected, differentiated into asexual forms (schizonts) only, almost all rapamycin-treated parasites (PBGAMi R+) transformed into gametocytes. We used the generated line to perform RNA-seq analysis of the R- and R+ populations at different time points of their development and identify the changes arising between them, mapping the sequence of events leading to the formation of gametocytes.

2018-06-30 | GSE110201 | GEO

Transcriptome changes during inducable gametocytogenesis in Plasmodium berghei parasites

2021-03-13 | GSE168817 | GEO

Transcriptome changes during inducible gametocytogenesis in Plasmodium berghei parasites

Project description:Transcriptome changes during inducible gametocytogenesis in Plasmodium berghei parasites

| PRJNA433164 | ENA

Plasmodium berghei Gametocytogenesis

Project description:Transcriptional profiling of gametocyte non-producer lines in Plasmodium berghei Transcriptome of gametocyte non producer lines (natural and genetic KO) and parental (820) lines. The aim of the study was to identify key genes involved in the decision to commit to gametocytogenesis in Plasmodium berghei. These microarrays compare naturally selected lines that do not produce gametocytes, and the parental line and additionally a genetic knock out of AP2-G PBANKA_143750. Data published Sinha, Hughes, et, al Nature tbc.

2013-12-13 | GSE53246 | GEO

Plasmodium berghei Gametocytogenesis

2013-12-13 | E-GEOD-53246 | biostudies-arrayexpress

Plasmodium berghei

Project description:Plasmodium berghei Gametocytogenesis

| PRJNA231246 | ENA

Global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites

Project description:The purpose of this research is to identify and evaluate the global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites and specifically compare the blood stage gene expression profiles of samples derived from previous studies on Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi

2016-04-08 | GSE80015 | GEO

Mechanisms of Triggering Malaria Gametocytogenesis by AP2-G [ChIP-seq]

Project description:AP2-G is a transcription factor (TF) that is essential for gametocytogenesis of malaria parasites; however, the mechanisms for its inducing the cell linage for sexual reproduction remain unclear. In this paper, this problem is addressed by investigating its expression profile and by determining target genes genome-wide in Plasmodium berghei.

2021-02-01 | GSE144970 | GEO

Mechanisms of Triggering Malaria Gametocytogenesis by AP2-G [RNA-seq]

2021-02-01 | GSE144965 | GEO

Mechanisms of Triggering Malaria Gametocytogenesis by AP2-G [DIP-seq]

2021-02-01 | GSE159045 | GEO