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Transcriptome RNA Sequencing Data Set of Differential Gene Expression in Escherichia Coli BW25113 SlyA Wild Type and Mutant Strains

ABSTRACT: Transcriptome RNA Sequencing Data Set of Differential Gene Expression in Escherichia Coli BW25113 SlyA Wild Type and Mutant Strains

PROVIDER: PRJNA714667 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13970433_1.fastq.gz	Fastqsanger.gz
	SRR13970433_2.fastq.gz	Fastqsanger.gz
	SRR13970434_1.fastq.gz	Fastqsanger.gz
	SRR13970434_2.fastq.gz	Fastqsanger.gz
	SRR13970435_1.fastq.gz	Fastqsanger.gz

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Transcriptome RNA Sequencing Data Set of Differential Gene Expression in Escherichia Coli BW25113 SlyA Wild Type and Mutant Strains

Project description:Escherichia coli laboratory strains remain instrumental to the discovery and development of biomarkers as drugs and diagnostic analytes in the post genomic era. The transcriptional regulator SlyA is a member of the multiple antibiotic resistance regulator family of transcription factors, which is associated with bacterial responses to host-derived oxidative stress, antibiotics resistance and virulence, and homologues exist in other Enterobacteriaceae. Here, we announce a transcriptome RNA sequencing data set detailing global gene expression in the wild type E. coli BW25113 and the slyA mutant. Results reveal heterogeneous functionality of SlyA that may vary between pathovars of E. coli. but which require further annotations of differentially expressed tRNAs

2021-03-16 | GSE168963 | GEO

Transcriptome RNA Sequencing Data Set of Differential Gene Expression in Escherichia coli BW25113 Wild-Type and <i>slyA</i> Mutant Strains.

Project description:Escherichia coli laboratory strains remain instrumental for the development of tools and techniques in molecular microbiology. The transcriptional regulator SlyA, associated with host-derived oxidative stress, antibiotic resistance, and virulence, is prominent in Enterobacteriaceae Here, we announce a transcriptome data set detailing the global gene expression in E. coli BW25113 and its slyA mutant.

| S-EPMC8142576 | biostudies-literature

E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells

Project description:This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells. Strains: E. coli K-12 BW25113 wild type, ompA mutant Medium: LB Cell type: Biofilm cells grown on glasswool and polystyrene surfaces Time: 15 h Temperature: 37C Strains: BW25113 ompA/pCA24N_ompA and ompA/pCA24N Medium: LB Time: 7 h Temperature: 37C Cell type: suspension cells, induced by 0.1 mM IPTG

2010-11-03 | E-GEOD-14064 | biostudies-arrayexpress

E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells

2010-01-20 | GSE14064 | GEO

BW25113 H202 and delta rpoS persister cells vs. stationary phase BW25113 wild-type

Project description:E. coli K-12 BW25113 persister cells generated via H202 pre-treatment and deletion of rpoS, relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL. Strains: BW25113 wild-type, pre-treated with H202 10 min, ampicillin 5 h; BW25113 delta rpoS, ampicillin 5 h; BW25113 wild-type OD at 600 nm of 3.0, ampicillin 30 mins. Medium: LB ampicillin 20 ug/mL used to generate persisters; ampicillin 5 ug/mL added to stationary phase BW25113 wild-type

2012-04-02 | E-GEOD-34028 | biostudies-arrayexpress

BW25113 Rif pre-treated persister cells vs. stationary phase BW25113 wild-type

Project description:E. coli K-12 BW25113 persister cells generated via rifampicin pre-treatment relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL.

2016-11-20 | GSE34177 | GEO

Whole transcriptome sequencing of E. coli BW25113 wt and nudC knockout strains

Project description:In the present study we performed total RNA sequencing of E. coli BW25113 wt and nudC knockout strains. RNA-seq analysis revealed that the inactivation of nudC gene in bacteria shows alteration of level of sRNA and protein-coding transcripts mainly associated with bacterial chemotaxis and flagellar assembly pathways.

2023-08-22 | GSE224606 | GEO

BW25113 H202 and delta rpoS persister cells vs. stationary phase BW25113 wild-type

2012-04-03 | GSE34028 | GEO

Transcriptional responses of Escherichia coli rpoS- BW25113 vs. wild-type BW25113 under 15% ethanol shock in log phase

Project description:RpoS, an alternative sigma factor, is critical for stress response in Escherichia coli.RpoS also acts as a global regulator for stress control of gene expression, and actually dose so in log stage and stationary stage. To further understand the effect of environmental stresses on in ethanologenic strains, DNA microarrys was used to analyze the expression profiles of E. coli and its rpoS mutant strain. BW25113（rpoS-）and BW25113 were selected at log stages and stationary stage of early development for RNA extraction and hybridization on Affymetrix microarrays. To that end, we hand-selected BW25113 and BW25113（rpoS-） according at different treatments: BW25113（rpoS-）at log stage (BW25113(rpoS-) log), BW25113 at log stage (BW25113 log),BW25113（rpoS-）atstationary stage ( BW25113(rpoS-) stationary), BW25113 at stationary stage ( BW25113 stationary)

2010-07-20 | E-GEOD-17526 | biostudies-arrayexpress

Transcription profiling of E. coli K12 Bw25113/pCA24N-hha with 2 mM IPTG vs BW25113/pCA24N with 2 mM IPTG

Project description:gene expression profiles by overexpressing Hha from pCA24N-hha using 2 mM IPTG induction in BW25113 wild type suspension cells in LB medium at 37C relative to BW25113 carrying the empty vector plasmid in the same test conditions. Experiment Overall Design: Strains: E. coli K12 BW25113/pCA24N, BW25113/pCA24N-hha Experiment Overall Design: Growth conditions: grow culture in LB medium at 37C to OD600 0.1 and add 2 mM IPTG to induce, and grow to OD600 0.5 to collect cells. RNA was extraction, and gene expression profiles were evaluated.

2008-10-20 | E-GEOD-10116 | biostudies-arrayexpress

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