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Gene expression during conidia germination in Neurospora crassa on PDA

ABSTRACT: Gene expression during conidia germination in Neurospora crassa on PDA

PROVIDER: PRJNA714875 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13977086_1.fastq.gz	Fastqsanger.gz
	SRR13977086_2.fastq.gz	Fastqsanger.gz
	SRR13977087_1.fastq.gz	Fastqsanger.gz
	SRR13977087_2.fastq.gz	Fastqsanger.gz
	SRR13977088_1.fastq.gz	Fastqsanger.gz

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Gene expression during conidia germination in Neurospora crassa on PDA

Project description:Conidia germination is critical for fungi to colonize various habitats. We sampled RNA expression at four stages of conidia germination, including fresh conidia (15min), polar growth (120min), doubling of long axis (240min), and first hyphal branching (360min) in Neurospora crassa. Cultures were made onPDA, iand two biological replicates were collected for all data points. The growth was under a labratory condition of 25C and constant light.

2022-03-17 | GSE168992 | GEO

Gene expression during conidia germination in Neurospora crassa on different media

Project description:Conidia germination is critical for fungi to colonize various habitats. We sampled RNA expression at four stages of conidia germination, including fresh conidia (15min), polar growth (120min), doubling of long axis (240min), and first hyphal branching (360min) in Neurospora crassa. Cultures were made on two different media, including Bird medium supporting only asexual development and maple sap medium supporting both asexual and sexual development, and two biological replicates were collected for all data points. The growth was under a labratory condition of 25C and constant light.

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Gene expression during conidia germination in Neurospora crassa on Bird medium at 37C

Project description:Conidia germination is critical for fungi to colonize various habitats. We sampled RNA expression at four stages of conidia germination, including fresh conidia (15min), polar growth (120min), doubling of long axis (240min), and first hyphal branching (360min) in Neurospora crassa. Cultures were made on Bird medium, and two biological replicates were collected for all data points. The growth was under a labratory condition of 375C and constant light.

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Gene expression during conidia germination in Neurospora crassa

Project description:Gene expression during conidia germination in Neurospora crassa

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Characterization of Neurospora crassa ghh (NCU06095) mutant strains

Project description:To determine the genes directly and indirectly under the control of the Grainy-head homolog (GHH) transcription factor in Neurospora crassa Three different sample types (Aerial Hyphae & Conidia; Mycelia; or Whole Colonies) of both wild-type (FGSC #2489) and grainy-head homolog (FGSC #13563) strains of Neurospora crassa were subjected to transcriptome analyses to determine the genes differentially expressed in the ghh background compared to wild type.

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Gene expression during conidia germination in Neurospora crassa on Bird medium at 37C

Project description:Gene expression during conidia germination in Neurospora crassa on Bird medium at 37C

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Genome-wide characterization of light-regulated genes in Neurospora crassa

Project description:RNA-seq from Neurospora crassa at 5 time points of light induction, with 2 replicates for each, totalling 10 samples RNA-seq from Neurospora crassa at 5 time points of light induction, with 2 replicates for each, totalling 10 samples

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Neurospora crassa

Project description:Gene expression during conidia germination in Neurospora crassa on different media

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Characterization of Neurospora crassa ghh (NCU06095) mutant strains

Project description:To determine the genes directly and indirectly under the control of the Grainy-head homolog (GHH) transcription factor in Neurospora crassa

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Evaluating the mode of Antifungal action of Heat-Stable Antifungal Factor (HSAF) in Neurospora crassa

Project description:Heat-stable antifungal factor (HSAF) isolated from Lysobacter enzymogenes has shown a broad-spectrum of antifungal activities. However, little is known about its mode of action. In this study, we used the model filamentous fungus Neurospora crassa to investigate the antifungal mechanism of HSAF. We first used HSAF to treat N. crassa strain for different time points. Spore germination, growth phenotype and differential gene expression analysis were conducted by utilizing global transcriptional profiling combined with genetic and physiological analyses. Our data showed that HSAF could significantly inhibit the germination and aerial hyphae growth of N.crassa. RNA-seq analysis showed a group of genes associated with cell wall formation and remodeling were highly activated. Screening of N. crassa gene deletion mutants combined with scanning electron microscopic observation revealed 3 fungal cell wall integrity related genes played important role in the interaction between N. crassa and L. enzymogens. In addition, WGCNA analysis, accompany with confocal microscopy observation revealed that HSAF could trigger autophagy mediated degradation and eventually result in cell death in N. crassa. The findings of this work provided new insights into the interactions between the predatory Lysobacter and its fungal prey.

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