Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA-Seq analysis of primed synovial fibroblasts

ABSTRACT: scRNA-Seq analysis of primed synovial fibroblasts

PROVIDER: PRJNA714968 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13981729.fastq.gz	Fastqsanger.gz
	SRR13981730.fastq.gz	Fastqsanger.gz
	SRR13981731.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

scRNA-Seq analysis of primed synovial fibroblasts

Project description:scRNA-Seq analysis of FACS sorted CD45-CD31- cells from naive and MSU crystals injected mouse paws.

2021-03-17 | GSE169051 | GEO

Transcriptomic analysis of primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse [RNA-seq]

Project description:Repeated challenge of synovial fibroblasts with MSU crystals in vivo induced inflammatory priming.

2021-03-17 | GSE169050 | GEO

Proteomic Analysis of the Interaction between Chicken Synovial Fibroblasts and Mycoplasma synoviae

Project description:Chicken synovial fibroblasts was infected without or with Mycoplasma synoviae for 48h

2019-11-29 | PXD016513 |

scRNA-seq with paired V(D)J sequencing of synovial B cells from rheumatoid arthritis patients

Project description:scRNA-seq with paired V(D)J sequencing of synovial B cells from rheumatoid arthritis patients.

2023-08-01 | E-MTAB-12833 | biostudies-arrayexpress

Transcriptomic analysis of primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse

Project description:Transcriptomic analysis of primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse

| PRJNA714965 | ENA

Transcriptomic analysis of cultured primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse (validation set of samples)

Project description:Repeated challenge of synovial fibroblasts with MSU crystals in vivo induced inflammatory priming

2020-12-24 | GSE163749 | GEO

Sequencing of dermal fibroblasts and synovial fibroblasts cultured in vitro

Project description:Knee osteoarthritis (KOA), as a degenerative multifactorial disease, affects the quality of life and mental health of patients, and also brings a huge socioeconomic burden. Treating synovitis have shown promise as anti-inflammatory therapeutics in mitigating OA symptoms and disease progression. Here, by analysing synovial single-cell sequencing (scRNA-seq) data from KOA, we found that synovial fibroblasts (FLS) in OA synovium showed a distinct pro-inflammatory phenotype. We collected synovial tissue from patients with clinical OA as well as from healthy donors, and histological examination was consistent with findings in scRNA-seq. Inspired by recent cross-tissue fibroblast lineage studies, we identified by sequencing that healthy FLS in synovial tissues share transcriptome-level similarities with dermal fibroblasts (DFb). Subsequently, we revealed the local as well as systemic distribution of intra-articular injected DFbs by constructing/extracting two types of rat fibroblasts (luciferase DFbs as well as GFP DFbs). The results demonstrate that DFbs can be locally retained in the synovium for up to three weeks following targeted engrafting on it. And intra-articular injection does not result in DFbs migration to vital organs or the occurrence of histological changes in these organs. A rat model of KOA was constructed by anterior cruciate ligament transection (ACLT) in order to study the therapeutic effect of DFbs on KOA. After injection, the rats showed improvement in painful gait. In addition, histological as well as imaging results showed reduced synovitis and improvement in articular cartilage. Finally we verified the protective effect of DFbs on cytokine-stimulated chondrocytes in a co-culture system.

2025-01-13 | GSE285940 | GEO

Transcriptomic analysis of primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse [RNA-seq]

Project description:Transcriptomic analysis of primed synovial fibroblasts from monosodium urate crystal-induced arthritis in the mouse [RNA-seq]

| PRJNA714967 | ENA

Transcriptional analysis of normal and pathological synovial fibroblasts

Project description:The aim of this study was to compare gene expression between two pathological groups of human synovial fibroblasts (SF) from rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissues with normal SF from healthy individuals (HSF). We used microarray expression profiling in SF cultured from OA, RA and normal synovial tissues. We found larger numbers of transcripts with differential expression in OASF compared to the other groups than in RASF compared to HSF. This data demonstrate that cultured OASF display a more robust transcriptomic profile than RASF when compared to HSF. Synovial fibroblasts were obtained from 9 patients with rheumatoid arthritis (RASF), 11 sex and age matched adult healthy donors (HSF) and 11 sex and age matched patients with OA (OASF). SF were collected under similar subconfluent conditions 24h after serum addition. 31 microarray data were used for determine the statistical significance (p value) of the differences in gene expression.

2011-10-24 | E-GEOD-29746 | biostudies-arrayexpress

Single-Cell RNA-Seq of Rheumatoid Arthritis Synovial Tissue

Project description:<p>Droplet-based single-cell RNA-seq has emerged as a powerful technique for massively parallel cellular profiling. While these approaches offer the exciting promise to deconvolute cellular heterogeneity in diseased tissues, the lack of cost-effective, reliable, and user-friendly instrumentation has hindered widespread adoption of droplet microfluidic techniques. To address this, we have developed a microfluidic control instrument that can be easily assembled from 3D printed parts and commercially available components costing approximately $575. We adapted this instrument for massively parallel scRNA-seq and deployed it in a clinical environment to perform single-cell transcriptome profiling of disaggregated synovial tissue from 5 rheumatoid arthritis patients. We sequenced 20,387 single cells from synovectomies, revealing 13 transcriptomically distinct clusters. These encompass a comprehensive and unbiased characterization of the autoimmune infiltrate, including inflammatory T and NK subsets that contribute to disease biology. Additionally, we identified fibroblast subpopulations that are demarcated via THY1 (CD90) and CD55 expression. Further experiments confirm that these represent synovial fibroblasts residing within the synovial intimal lining and subintimal lining, respectively, each under the influence of differing microenvironments. We envision that this instrument will have broad utility in basic and clinical settings, enabling low-cost and routine application of microfluidic techniques, and in particular single-cell transcriptome profiling.</p> <p>Reprinted from [Stephenson et al., Nature Communications, 2018], with permission from the Nature Publishing Group.</p>

| phs001529 | dbGaP

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data