Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Replicative history marks transcriptional and functional disparity in the memory T cell pool

ABSTRACT: Replicative history marks transcriptional and functional disparity in the memory T cell pool

PROVIDER: PRJNA715447 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13997740_1.fastq.gz	Fastqsanger.gz
	SRR13997740_2.fastq.gz	Fastqsanger.gz
	SRR13997741_1.fastq.gz	Fastqsanger.gz
	SRR13997741_2.fastq.gz	Fastqsanger.gz
	SRR13997742_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 96

Similar Datasets

Replicative history marks transcriptional and functional disparity in the memory T cell pool II

Project description:Replicative history marks transcriptional and functional disparity in the memory T cell pool II

| PRJNA767084 | ENA

Replicative history marks transcriptional and functional disparity in the memory T cell pool II

Project description:To validate whether division history is linked to functional and transcriptional properties we established a CTV-based serial-transfer approach that allowes single cell transcriptional profiling of memory T cells that have undergone a high or low amount of prior division

2022-01-31 | GSE184947 | GEO

Replicative history marks transcriptional and functional disparity in the memory T cell pool

Project description:Mice received DivisionRecorder+ or unmodified OT-I T cells and were challenged with LM-OVA. During memory phase we FACS sorted memory CD8+ OT-I T cells (and sub-sorted RFP+ and RFP- cells in the case of DivisionRecorder+ cells) from spleen material and performed single cell RNA sequencing.

2021-11-01 | GSE169154 | GEO

Replicative history marks transcriptional and functional disparity in the CD8<sup>+</sup> T cell memory pool.

Project description:Clonal expansion is a core aspect of T cell immunity. However, little is known with respect to the relationship between replicative history and the formation of distinct CD8+ memory T cell subgroups. To address this issue, we developed a genetic-tracing approach, termed the DivisionRecorder, that reports the extent of past proliferation of cell pools in vivo. Using this system to genetically 'record' the replicative history of different CD8+ T cell populations throughout a pathogen-specific immune response, we demonstrate that the central memory T (TCM) cell pool is marked by a higher number of prior divisions than the effector memory T cell pool, owing to the combination of strong proliferative activity during the acute immune response and selective proliferative activity after pathogen clearance. Furthermore, by combining DivisionRecorder analysis with single-cell transcriptomics and functional experiments, we show that replicative history identifies distinct cell pools within the TCM compartment. Specifically, we demonstrate that lowly divided TCM cells display enriched expression of stem-cell-associated genes, exist in a relatively quiescent state, and are superior in eliciting a proliferative recall response upon activation. These data provide the first evidence that a stem-cell-like memory T cell pool that reconstitutes the CD8+ T cell effector pool upon reinfection is marked by prior quiescence.

| S-EPMC7612726 | biostudies-literature

Replicative senescence of mesenchymal stem cells causes DNA methylation changes which correlate with repressive histone marks

Project description:Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long‐term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony‐ forming unit (CFU‐f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP‐microarrays. Subsequently, we have compared DNA‐methylation profiles with the Infinium HumanMethylation27 Bead Array and the profiles differed markedly in MSC derived from adipose tissue and bone marrow. Notably, all MSC revealed highly consistent senescence‐associated modifications at specific CpG sites. These DNA‐methylation changes correlated with histone marks of previously published data sets, such as trimethylation of H3K9, H3K27 and EZH2 targets. Taken together, culture expansion of MSC has profound functional implications ‐ these are hardly reflected by genomic instability but they are associated with highly reproducible DNA‐methylation changes which correlate with repressive histone marks. Therefore replicative senescence seems to be epigenetically controlled. 8 samples of mesenchymal stem cells (MSC) from human adipose tissue

2011-10-27 | E-GEOD-26519 | biostudies-arrayexpress

Replicative senescence of mesenchymal stem cells causes DNA methylation changes which correlate with repressive histone marks

2011-10-28 | GSE26519 | GEO

Transcriptomic responses to phosphate limitation in maize lines with different breeding history

Project description:Plants cope with low phosphorus availability by adjusting growth and metabolism through transcriptomic adaptations. We hypothesize that selected genotypes with distinct P use efficiency covering the breeding history of European heterotic pool allow us to reveal general and genotype-specific molecular responses correlated with low phosphate induced traits.

2022-04-23 | GSE201119 | GEO

brine pool

Project description:microbiology of brine pool

| PRJEB12415 | ENA

CHINA MTB HISTORY

Project description:CHINA MTB HISTORY

| PRJEB23157 | ENA

Pool-Seq mappers

Project description:Pool-Seq mappers

| PRJEB13602 | ENA