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Duodenal Mucosal Barrier in Functional Dyspepsia

ABSTRACT: Duodenal Mucosal Barrier in Functional Dyspepsia

PROVIDER: PRJNA716241 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14029229_1.fastq.gz	Fastqsanger.gz
	SRR14029229_2.fastq.gz	Fastqsanger.gz
	SRR14029230_1.fastq.gz	Fastqsanger.gz
	SRR14029230_2.fastq.gz	Fastqsanger.gz
	SRR14029231_1.fastq.gz	Fastqsanger.gz

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Duodenal Mucosal Barrier in Functional Dyspepsia [miRNA]

Project description:Duodenal Mucosal Barrier in Functional Dyspepsia [miRNA]

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Duodenal Mucosal Barrier in Functional Dyspepsia [mRNA]

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Duodenal Mucosal Barrier in Functional Dyspepsia [miRNA]

Project description:Epithelial Barrier and Leaky Gut in Dyspepsia Running title: Epithelial Barrier and Leaky Gut in Dyspepsia Context: Some studies suggest that FD is associated with ex vivo duodenal epithelial micro-inflammation and barrier impairment; the pathogenesis of these findings is unclear. miRNAs reduce expression of epithelial barrier genes and have been postulated to increase epithelial permeability in irritable bowel syndrome. New findings: Compared to controls, there is reduced mRNA expression of several barrier proteins (zona occludin-1), increased expression of several miRNAs (eg, miR-142-3p) that suppress the genes for barrier proteins in FD. However, mucosal eosinophils, intraepithelial lymphocytes, and mast cells, ex- and in vivo permeability (urinary lactulose and mannitol excretion) were not significantly different in FD. Impact: Patients with FD do not have a leaky gut syndrome.

2023-03-01 | GSE169307 | GEO

Duodenal Mucosal Barrier in Functional Dyspepsia [mRNA]

2023-03-01 | GSE169304 | GEO

Duodenal Mucosal Barrier in Functional Dyspepsia.

Project description:Background & aimsIn addition to gastric sensorimotor dysfunctions, functional dyspepsia (FD) is also variably associated with duodenal micro-inflammation and epithelial barrier dysfunction, the pathogenesis and clinical significance of which are unknown. Our hypothesis was that miRNAs and/or inflammation degrade epithelial barrier proteins, resulting in increased duodenal mucosal permeability in FD.MethodsWe compared the duodenal mucosal gene expression and miRNAs, in vivo permeability (lactulose-mannitol excretion between 0 and 60 and 60 and 120 minutes after saccharide ingestion), ex vivo assessments (transmucosal resistance, fluorescein isothiocyanate [FITC]-dextran flux, and basal ion transport), and duodenal histology (light and electron microscopy) in 40 patients with FD and 24 controls.ResultsCompared with controls, the mRNA expression of several barrier proteins (zonula occludens-1, occludin, claudin-12, and E-cadherin) was modestly reduced (ie, a fold change of 0.8-0.85) in FD with increased expression of several miRNAs (eg, miR-142-3p and miR-144-3-p), which suppress these genes. The urinary lactulose excretion and the lactulose:mannitol ratio between 60 and 120 minutes were greater in FD than in controls (P < .05). The FITC-dextran flux, which reflects paracellular permeability, was inversely correlated (r = -0.32, P = .03) with transmucosal resistance and directly correlated (r = 0.4, P = .02) with lactulose:mannitol ratio. Other parameters (mucosal eosinophils, intraepithelial lymphocytes, and mast cells, transmucosal resistance, FITC-dextran flux, average intercellular distance, and proportion of dilated junctions) were not significantly different between groups.ConclusionsIn FD, there is a modest reduction in the expression of several duodenal epithelial barrier proteins, which may be secondary to up-regulation of regulatory miRNAs, and increased small intestinal permeability measured in vivo.

| S-EPMC8975906 | biostudies-literature

Duodenal Mucosal Barrier in Functional Dyspepsia

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-01 | GSE169308 | GEO

Proteomic analysis of the impact of Jianwei Xiaoshi tablets on rats with functional dyspepsia of spleen deficiency

Project description:Proteomic analysis was conducted on gastric and duodenal tissues from rats with functional dyspepsia of spleen deficiency and normal rats, as well as rats with functional dyspepsia treated with Jianwei Xiaoshi tablets.

2023-11-29 | PXD047366 |

Chemical profiling of Jianwei Xiaoshi oral liquid and its potential mechanism of effect on high-calorie diet-induced dyspepsia in immature rats: gut flora, transcriptomics and network pharmacology

Project description:Gene expression was detected using duodenal samples to identify the key regulatory genes for the treatment of dyspepsia with Jianwei Xiaoshi oral liquid

2023-12-31 | GSE245835 | GEO

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia.

Project description:ObjectivesThe pathophysiology of functional dyspepsia (FD) is not fully understood. Impaired duodenal mucosal integrity characterized by increased mucosal permeability and/or low-grade inflammation was reported as potentially important etiologies. We aimed to determine the utility of a recently developed simple catheterization method to measure mucosal admittance (MA), the inverse of mucosal impedance, for evaluation of duodenal mucosal permeability in patients with FD.MethodsWe conducted two prospective studies. In the first study, duodenal MA of 23 subjects was determined by catheterization during upper endoscopy, and transepithelial electrical resistance (TEER) of duodenal biopsy samples in Ussing chambers was measured to assess the correlation between MA and TEER. In the second study, duodenal MA of 21 patients with FD fulfilling the Rome III criteria was compared with that of 23 healthy subjects.ResultsThe mean MA and TEER values were 367.5±134.7 and 24.5±3.7 Ω cm2, respectively. There was a significant negative correlation between MA and TEER (r=-0.67, P=0.0004, Pearson's correlation coefficient). The mean MA in patients with FD was significantly higher than that in healthy subjects (455.7±137.3 vs. 352.1±66.9, P=0.002, unpaired t-test). No procedure-related complications were present.ConclusionsWe demonstrated the presence of increased duodenal mucosal permeability in patients with FD by MA measurement using a simple catheterization method during upper endoscopy.

| S-EPMC5415894 | biostudies-literature

Evaluation of transcriptional changes in the duodenal tissue during acute pancreatitis

Project description:mRNA was isolated from duodenal tissue 3d after induction of acute pancreatitis in C57Bl/6 wild type mice to investigate transcriptional changes, untreated C57Bl/6 refer as controls we used microarrays to investigate the duodenal barrier function during acute pancreatitis

2023-02-01 | GSE196411 | GEO

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