Project description:Select five different treatment groups, including fifth instar larvae, pre diapause larvae, diapause larvae, diapause release treated larvae, and post diapause released larvae, for proteomic analysis
Project description:Transcriptome analysis (RNA-Seq) was performed to identify Kr-h1 target genes. Message RNAs were extracted from wildtype and kr-h1[7] homozygous 3rd instar larvae at 100 hr after egg laying (two biological replicates for each genotype).
Project description:Affymetrix microarray to detect changes in gene expression between lgl27S3/lglE2S31 and FRT82B larvae 4-day old 3rd instar lgl27S3/lglE2S31 larvae were compared to 0-day old 3rd instar FRT82B larvae
Project description:Single channel custom oligonucleotide array of 147 microRNAs to detect changes in expression of a lgl-hypomorph mutant versus wild-type 0, 3, and 5 day old 3rd instar lgl-hypomorph larvae were compared to 0 day 3rd instar wild-type larvae to test for differences in microRNA expression
