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RNA-seq analysis of HCT116 human colon cancer cell line silenced ID4 expression

ABSTRACT: RNA-seq analysis of HCT116 human colon cancer cell line silenced ID4 expression

PROVIDER: PRJNA717016 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14062690_1.fastq.gz	Fastqsanger.gz
	SRR14062690_2.fastq.gz	Fastqsanger.gz
	SRR14062691_1.fastq.gz	Fastqsanger.gz
	SRR14062691_2.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of HCT116 human colon cancer cell line silenced ID4 expression

Project description:To investigate the function of ID4 in colon cancer cells, we collected total RNA from both HCT116-SC and HCT116-shID4 and examined mRNA expression profile, comprehensively.

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Human colon cancer cell line HCT116 stably transfected with EPXH2 was constructed and analyzed by RNA sequencing.

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High-throughput RNA sequencing analysis of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to evaluate the effects of si-LINC00152 on the mRNA of human colon cancer cell line HCT116. Methods: Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting LINC00152 for 36 hours in DMEM medium (with 10% serum). Total RNA were extracted and detected by Illumina high-throughput RNA sequencing data analysis. 2 independent biological replicates were plated, transfected in parallel for each control and siRNA. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 90 mRNAs were up-regulated and 159 were down-regulated in “si-LINC00152” group comparing to “control” group. Conclusions: Our study describes the mRNA changes of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.

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mRNA expression of HUVEC cells treated by exosomes derived from colorectal cancer cell line HCT116 transfected with lncRNA-APC1 silenced or control vector

Project description:To determine the mRNA expression profile of HUVEC cells treated by exosomes derived from colorectal cancer cell line HCT116 transfected with lncRNA-APC1 silenced or control vector, we performedd gene expression microArray analysis form Arraystar to examine the expression of mRNAs.

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Expression data for human colon cancer cell line HCT116 transfected with siRNA targeting YAP1.

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