Project description:The mammalian brain is complex, with multiple cell types performing a variety of diverse functions, but exactly how each cell type is affected in aging remains largely unknown. Here we performed a single-cell transcriptomic analysis of young and old mouse brains. We provide comprehensive datasets of aging-related genes, pathways and ligand–receptor interactions in nearly all brain cell types. Our analysis identified gene signatures that vary in a coordinated manner across cell types and gene sets that are regulated in a cell-type specific manner, even at times in opposite directions. These data reveal that aging, rather than inducing a universal program, drives a distinct transcriptional course in each cell population, and they highlight key molecular processes, including ribosome biogenesis, underlying brain aging. Overall, these large-scale datasets provide a resource for the neuroscience community that will facilitate additional discoveries directed towards understanding and modifying the aging process.

2019-07-01 | GSE129788 | GEO

Single-cell transcriptomic profiling of the aging mouse brain

Project description:Single-cell transcriptomic profiling of the aging mouse brain

| PRJNA532831 | ENA

Single cell analysis of the aging hypothalamus

Project description:Single cell analysis of the aging hypothalamus

| PRJNA779749 | ENA

Single cell analysis of the aging hypothalamus

Project description:Alterations in metabolism, sleep patterns, body composition, and hormone status are all key features of aging. The hypothalamus is a well-conserved brain region that controls these homeostatic and survival-related behaviors. Despite the importance of this brain region in healthy aging, little is known about the intrinsic features of hypothalamic aging. Here, we utilize single nuclei RNA-sequencing to assess the transcriptomes of 40,064 hypothalamic nuclei from young and aged female mice. We identify cell type-specific signatures of aging in neurons, astrocytes, and microglia, as well as among the diverse collection of neuronal subtypes in this region. We uncover key changes in cell types critical for metabolic regulation and body composition, as well as in an area of the hypothalamus linked to cognition. In addition, our analysis reveals female-specific changes in sex chromosome regulation in the aging brain. This study identifies critical cell-specific features of the aging hypothalamus in mammals.

2022-05-20 | GSE188646 | GEO

Transcriptomic signatures of the aging brain at single-cell resolution

Project description:Transcriptomic signatures of the aging brain at single-cell resolution

| PRJNA742781 | ENA

Single-Cell Analysis of Aging Mouse Dentate Gyrus

Project description:Single-Cell Analysis of Aging Mouse Dentate Gyrus

| PRJNA848388 | ENA