Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sus scrofa

ABSTRACT: Raw sequence reads of transcriptome study of different tissues of African swine fever infection

PROVIDER: PRJNA719293 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14141500_1.fastq.gz	Fastqsanger.gz
	SRR14141500_2.fastq.gz	Fastqsanger.gz
	SRR14141501_1.fastq.gz	Fastqsanger.gz
	SRR14141501_2.fastq.gz	Fastqsanger.gz
	SRR14141487_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

African swine fever virus tropism for different organ tissues and host transcriptomic responses in experimentally infected pigs

Project description:Purpose: This goal of this study was to explore the host transcriptomic responses in African swine fever virus experimentally infected pigs using RNA-Sequencing. Methods: RNAs acquired from ten different organ tissue samples were sequenced. Sequencing reads were preprocessed, aligned with the reference genome, assembled and expressions were estimated through bioinformatics approaches. Result: Several uprugulated DEGs were identified. Conclusion: We found important candidate genes and pathways for further testing in African swine fever virus infection in pig.

2023-04-23 | GSE230340 | GEO

the analysis of sus scrofa serum LC-MS/MS after african swine fever virus infection

Project description:African swine fever (ASF) is the most dangerous disease of pigs and causes enormous economic losses in the global pig industry. However, the mechanism of ASF virus (ASFV) infection is unclear. Hence, we wanted to understand the host response mechanism upon ASFV infection. We analyzed the differentially expressed proteins (DEPs) between ASFV-infected and un-infected serum samples using quantitative proteomics. Setting the p-value < 0.05 and |log2 (fold change)| > 1.5, we identified 173 DEPs, including 57 upregulated and 116 downregulated proteins, which belonged to various biological processes and pathways according to the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. The enriched pathways include the immune system, metabolism, and inflammation.

2022-10-12 | PXD035545 | Pride

Homo sapiens

Project description:Raw sequence reads of transcriptome study of different tissues of African swine fever infection

| PRJNA719275 | ENA

Sus scrofa domesticus

Project description:The African Suidae genome architectures shed light on the evolution of natural reservoirs of African swine fever virus

| PRJNA1057267 | ENA

Comparison of the proteomes of porcine macrophages and a stable porcine cell line after infection with African swine fever virus

Project description:Quantitiative analysis of proteomes of porcine macrophages and the stable cell line WSL infected with African swine fever virus using a nanoLC MALDI-Tof/Tof MS platform. The impact of the infection on the two cell types was analyzed using Gene Ontology term and KEGG pathway enrichment analysis.

2021-10-26 | PXD028838 | JPOST Repository

Evaluation of the African swine fever virus-induced host shutoff using dynamic stable isotope labeling with amino acids in cell culture (SILAC)

Project description:African swine fever is a viral disease of swine caused by the African swine fever virus (ASFV). Currently, ASFV is a serious threat to the global pig industry. A viral strategy to undermine host cell response is to establish a global shutoff of host protein synthesis (virus-induced shutoff, vhs). Here, we characterize ASFV-induced shutoff in primary porcine macrophages by measurement of relative protein synthesis rates based on stable isotope labeling with amino acids in cell culture (SILAC). The impact of ASFV infection on the synthesis of >2000 individual host proteins showed a high degree of variability ranging from complete shutoff to a strong induction of proteins that are absent from naïve cells. By GO-term enrichment analysis the cellular pathways that were most efficiently impacted by vhs were identified. The experimental setup is suitable to quantify vhs after infections with different viruses.

2023-05-31 | PXD039806 | Pride

RNA-seq of early responses to ASFV

Project description:In this project we would like to study immune responses to African swine fever virus in pigs. We have realized a large animal experiment using two different viruses and pigs with different immune status. We have collected paxgene blood RNA tubes in order to investigate transcriptional changes at different stages of the early immune response.

2022-07-06 | E-MTAB-11634 | biostudies-arrayexpress

The effect of African Swine Fever Virus infection on porcine host microRNAs

Project description:Large DNA viruses are known to manipulate and modify host miRNAs during infection. Therefore the aim of this study was to investigate the impact of infection with the large DNA virus; African swine fever virus (ASFV) on host miRNAs. Small RNA sequencing libraries were prepared from RNA extracted from ASFV (Benin 97/1) infected primary porcine macrophages at 0, 6 and 16 hours post infection. Libraries were pooled and sequenced on 1 lane of an Illumina HiSeq, yielding sequences aligning to a total of 247 different mature Sus scrofa miRNAs. On average, 3779095 (± 1911525) miRNA reads were obtained per sample. The results revealed no widespread modification to host miRNAs, though a number of specific miRNAs were differentially expressed during ASFV infection. Notably, a small number of miRNAs (Ssc-miR-10b, Ssc-miR-144 and Ssc-miR-486) were rapidly upregulated 2-6 fold within the first hour of infection.

2020-07-28 | GSE115512 | GEO

Sus scrofa

Project description:Transcriptome analysis of swine testis cells following swine transmissible gastroenteritis coronavirus infection

| PRJNA318729 | ENA

Sus scrofa

Project description:Response of swine lung to H1N1 swine influenza virus infection revealed by transcription analysis

| PRJNA140591 | ENA