Project description:Tellurium (Te) is a metalloid that is growing as a challenge since its increasing environmental presence, as a result of mining activity and improper disposal of high-tech devices, leads to an increasing selective pressure against living organisms. How microorganims adapt to the presence of increasing concentrations of metalloids, such as Te, could increase the know-how to solve Te contamination problems. Two mining isolates, Bacillus sp. and Paenibacillus sp, were able to grow in high concentrations of Te(IV) and were able to reduce Te(IV) to Te(0) so the effects of growth in the presence of Te(IV) was determined, at the level of total protein expression, for this strains. It was determined that growth of these strains in the presence of Te(IV) affected the general protein expression pattern, and hence the metabolism.
Project description:Transcriptional profiling of the bacteria Paenibacillus vortex comparing control untreated cells with kanamycin treated cells after 18 hours of exposure. Goal was to determine the effect of the antibiotic kanamycin in concentration which affect the colony morphology on global bacteria gene expression.
Project description:Paenibacillus polymyxa is a root-associated plant growth-promoting rhizobacterium. It was reported that many strains of P. polymyxa naturally exhibited the phenotypic variation. In the phase variation, the characteristics of the wild-type ‘B’ and the variant ‘F’ are very different in sporulation formation, motility, antibiotic ability and so on. For better understanding of the actual physiological changes, we performed RNA-seq analyses of P. polymyxa E681 to compare genome wide patterns of gene expression. As a result, we obtained 1,062 differentially expressed genes related to flagellar assembly and transport systems.
